大熊猫与亚洲黑熊的红细胞膜蛋白质谱检测  

Mass Spectrometric Detection of Red Cell Membrane Proteins in Giant Pandas and Asian Black Bears

作　　者：邓林华 赵俸涌[2] 李勤[2] 黄山 魏明 张亚辉 王承东 李德生 朱自严[2] DENG Linhua;ZHAO Fengyong;LI Qin;HUANG Shan;WEI Ming;ZHANG Yahui;WANG Chengdong;LI Desheng;ZHU Ziyan(China Conservation and Research Center for the Giant Panda,Key Laboratory of National Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park,Dujiangyan,611830,China;Shanghai Blood Center,Shanghai,200051,China)

机构地区：[1]中国大熊猫保护研究中心,大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室,都江堰611830 [2]上海市血液中心,上海200051

出　　处：《野生动物学报》2021年第1期37-43,共7页CHINESE JOURNAL OF WILDLIFE

基　　金：大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室(中国大熊猫保护研究中心)开放基金(KLSFGAGP2020.018);中国大熊猫保护研究中心科研年项目(CCRCGP181933);香港海洋公园保育基金(TM02_1920)。

摘　　要：熊科动物分布于我国的各个地区,不同的生存条件与生活习性导致其机体生理生化存在差异。红细胞作为气体运输的重要载体,对机体代谢及生命学过程有重要作用。本研究中,通过流式分选技术对大熊猫及亚洲黑熊红细胞进行纯化,并通过液相色谱偶联二级质谱的方法,对大熊猫及亚洲黑熊红细胞膜蛋白进行检测。通过检测,同检测到大熊猫红细胞膜蛋白388种,亚洲黑熊红细胞膜蛋白315种,并对上述检测中膜蛋白功能进行分类检索,发现二者红细胞膜蛋白主要功能较为一致,但也发现大熊猫参与能量代谢的膜蛋白略多于亚洲黑熊。Representatives of the Ursidae are distributed in various regions of China.Different living conditions and habits lead to differences in their physiology and biochemistry.As an important transporter of gases,red blood cells play an important role in mammalian metabolism and biotic processes.In this study,giant panda(Ailuropoda melanoleuca)and Asian black bear(Ursus thibetanus)red blood cells were purified by flow cytometry,and the red cell membrane proteins were detected by liquid chromatography coupled with two-phase mass spectrometry.Through testing,388 types of red cell membrane proteins of giant panda and 315 types of black cell membrane proteins of Asian black bear were detected.We classified and searched membrane protein functions.The main functions of red cell membrane proteins were more consistent,but slightly more membrane proteins were involved in energy metabolism in giant panda than in Asian black bear.

关 键 词：大熊猫 亚洲黑熊 红细胞 膜蛋白 代谢 

分 类 号：S852[农业科学—基础兽医学]