基于罗丹明-高半胱氨酸荧光探针对Hg^2+的检测  

Detection of Hg^2+ with a Rhodamine-Homocysteine Fluorescent Probe

作　　者：李爱玲 赵秋媛 王红斌 周丽波 张艳丽 谭伟 张丽珠 LI Ailing;ZHAO Qiuyuan;WANG Hongbin;ZHOU Libo;ZHANG Yanli;TAN Wei;ZHANG Lizhu(School of Chemistry and Environment,Yunnan Minzu University,Kunming 650500;School of Nationalities Medicine,Yunnan Minzun University,Kunming 650500)

机构地区：[1]云南民族大学化学与环境学院,云南昆明650500 [2]云南民族大学民族医药学院,云南昆明650500

出　　处：《分析科学学报》2020年第6期895-899,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21665027);国家大学生创新创业项目(No.201710691004);云南民族大学研究生创新基金项目(No.2018YJCXS246;2018YJCXS248)。

摘　　要：在罗丹明衍生物(Rh6G2)中引入高半胱氨酸(Hcy),通过自组装反应,制备了一种新型的荧光探针(Hcy-Rh6G2),建立了一种对Hg^2+的荧光检测方法。考察了探针Hcy-Rh6G2的荧光光谱性能及其对常见离子的选择性。结果表明:在甲醇和4-羟乙基哌嗪乙磺酸(HEPES)缓冲体系中,探针Hcy-Rh6G2可在较宽的pH范围内(pH=6.2~12.0)高灵敏、高选择性地检测Hg2+。Hg^2+浓度在2.50×10^-5~2.25×10^-4 mol/L的范围内具有良好的线性关系,检出限为6.19×10^-6 mol/L,而且方法受常见金属阳离子和阴离子干扰较小。实际水样测定的平均回收率为97.0%~98.9%,相对标准偏差(RSD)在0.34%~0.57%之间。A novel fluorescent probe(Hcy-Rh6G2)was prepared by self-assembly reaction using rhodamine derivatives(Rh6G2)and homocysteine(Hcy)as raw materials,and a fluorescence detection method for Hg 2+was established.The fluorescence properties of the probe Hcy-Rh6G2 and its influencing factors were investigated.The results show that in the mixture of methanol and 4-hydroxyethylpiperazineethanesulfonic acid(1∶1,V/V),the probe Hcy-Rh6G2 can detect Hg^2+with high sensitivity and selectivity in a wide pH range(pH=6.2-12.0).The Hg^2+concentration has a good linear relationship in the range of 2.50×10-5-2.25×10^-4 mol/L.The detection limit is 6.19×10^-6 mol/L,which is less disturbed by common metal cations and anions.The average recoveries of actual water samples are 97.0%-98.9%,RSDs are 0.34%-0.57%.

关 键 词：荧光探针 高半胱氨酸 罗丹明 HG^2+ 

分 类 号：O657.39[理学—分析化学]