基于SSR分子标记检测褐飞虱交配次数及性选择  

Detection of Mating Times and Sexual Selection of Brown Planthopper(Nilaparvata Lugens St l)Based on SSR Molecular Markers

作　　者：荆胜利 郑新静 李亚楠 张丽 余青青 黄小燕 杜波 杨芳 刘清松 JING Shengli;ZHENG Xinjing;LI Yanan;ZHANG Li;YU Qingqing;HUANG Xiaoyan;DU Bo;YANG Fang;LIU Qingsong(College of Life Sciences,Xinyang Normal University,Xinyang 464000,China;College of Life Sciences/National Key Laboratory of Hybrid Rice,Wuhan University,Wuhan 430072,China)

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000 [2]武汉大学生命科学学院/杂交水稻国家重点实验室,湖北武汉430072

出　　处：《信阳师范学院学报（自然科学版）》2021年第1期42-49,共8页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(U1704111,31901896,31401732);杂交水稻国家重点实验室(武汉大学)开放课题基金项目(KF201606);植物病虫害生物学国家重点实验室开放课题项目(SKLOF201701和SKLOF201818);信阳师范学院“南湖学者奖励计划”青年项目。

摘　　要：为探明褐飞虱交配次数和性选择的生殖行为特征,构建了褐飞虱生物型1和生物型Y的近交系,筛选了在不同近交系之间存在明显差异的9个SSR(Simple Sequence Repeats)分子标记用于亲子鉴定.在雌虫交配次数试验中,观察并分子鉴定到23头成功交配的雌虫,其中19头雌虫一生仅进行1次交配,4头雌虫一生进行了2次交配,故褐飞虱雌虫是以单次交配为主、2次交配为辅的混合型交配策略.在性选择分析中,首先,性别内选择试验观察并鉴定到了23头交配成功的雄虫,其中18头生物型Y雄虫选择与雌虫进行交配;其次,性别间选择试验观察并鉴定到了83%生物型Y雄虫选择与雌虫进行交配.结果表明:在性选择行为中,生物型Y雄虫具有更强的竞争优势.In order to investigate the reproductive behavior characteristics of the mating times and sexual selection of brown planthopper(Nilaparvata lugens St l),the inbred lines of brown planthopper biotype 1 and biotype Y were constructed,and 9 SSR(Simple Sequence Repeats)molecular markers with obvious size differences between the two lines were selected for paternity identification.In the female mating times experiment,23 mating females were successfully observed and molecularly identified.Among them,19 females mated only once along their lifetime,and only 4 females mated two times along their lifetime.Thus,there is a mixed mating strategy for brown planthopper female with mainly basing on single mating and supplementing by mating twice during its whole lifetime.In the sexual selection analysis,firstly,23 mating males were successfully observed and identified in the intra-sex selection experiment,among which 18 biotype Y males choosed to mate with females;secondly,83%biotype Y males that observed and identified in the inter-sex selection experiment choose to mate with females.Taken together,these results suggest that the biotype Y male has a stronger competitive advantage in sexual selection.

关 键 词：褐飞虱 微卫星分子标记 亲子鉴定 交配次数 性选择 

分 类 号：Q38[生物学—遗传学]