菠菜叶酸合成代谢途径基因鉴定及表达谱分析  

Gene identification and expression profiling analysis of spinach folate anabolic pathway

作　　者：张玖漪 蔡晓锋[1,2] 徐晨曦[1,2] 王全华[1,2] 王小丽[1,2] ZHANG Jiuyi;CAI Xiaofeng;XU Chenxi;WANG Quanhua;WANG Xiaoli(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Development Center of Plant Germplasm Resources,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海师范大学植物种质资源开发协同创新中心,上海200234

出　　处：《上海师范大学学报（自然科学版）》2020年第6期637-649,共13页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金青年基金(31601744);上海植物种质资源工程技术研究中心项目(17DZ2252700);上海市绿叶菜产业技术体系项目(沪农科产字(2018)第2-06字)。

摘　　要：从菠菜(Spinacia oleracea L.)基因组数据库中筛选鉴定了与菠菜叶酸合成转运,及与C1代谢相关的25个基因,并对其编码的蛋白做进化树和保守域分析,发现叶酸蛋白在进化上表现出保守型和复杂性。用实时荧光定量聚合酶链式反应(qRT-PCR)分析了叶酸含量不同的菠菜的叶酸相关基因表达量,发现在转录水平上只有少数叶酸基因表达量与菠菜叶酸含量有关。In this paper,25 genes related to spinach’s(Spinacia oleracea L.)folate synthesis and transport,and to C1 metabolism were identified in the spinach genome database.The evolutionary tree and conserved domain analysis of the encoded protein showed that the folate protein showed conservative type and complexity in evolution.Using the technology of quantitative real-time polymerase chain reaction(qRT-PCR)to analyze the expression of folate-related genes in spinach with different folate content,it was found that only a few folate gene expression levels were related to the content of spinach folate at the transcription level.

关 键 词：菠菜(Spinacia oleracea L.) 叶酸 基因表达 

分 类 号：S636.1[农业科学—蔬菜学] Q563.8[农业科学—园艺学]