两种黄芪剂量补阳还五汤对海绵体神经损伤性大鼠勃起功能障碍的治疗效果  

Effect of the Astragalus dose in Buyang Huanwu decoction on erectile dysfunction in rats with cavernous nerve injury

作　　者：周康 叶妙勇 赵凡 马轲 吕伯东 许增宝[7] ZHOU Kang;YE Miaoyong;ZHAO Fan;MA Ke;LYU Bodong;XU Zengbao(Second School of Clinical Medicine,Zhejiang University of Chinese Medicine,Hangzhou 310053,China;Department of Urology,Wenling First People’s Hospital,Wenling 317500;Department of Urology and Andrology,the Affiliated Hospital of Nantong University,Nantong 226001;Department of Urology,the Second Hospital Affiliated to Zhejiang Chinese Medicine University,Hangzhou 310005;Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine/Laboratory of Andrology,Hangzhou 310053;Research Institute of Urology and Andrology,Zhejiang University of Chinese Medicine,Hangzhou 310005;Huzhou Hospital of Traditional Chinese Medicine,Huzhou 313000)

机构地区：[1]浙江中医药大学第二临床医学院,杭州310053 [2]温岭市第一人民医院泌尿外科,浙江温岭317500 [3]南通大学附属医院泌尿外科/男科,江苏南通226001 [4]浙江中医药大学附属第二医院泌尿男科,杭州310005 [5]浙江省中医药重点实验室/男科实验室,杭州310053 [6]浙江中医药大学泌尿男科研究所,杭州310005 [7]湖州市中医院,浙江湖州313000

出　　处：《中国实验动物学报》2020年第6期788-795,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：浙江省自然科学基金(LY18H270002)。

摘　　要：目的探究两种黄芪剂量补阳还五汤对海绵体神经损伤性大鼠勃起功能障碍的治疗效果。方法将32只SD大鼠随机平均分成假手术组、模型组、30 g组(模型+含黄芪30 g补阳还五汤)、120 g组(模型+黄芪120 g补阳还五汤);通过手术对SD大鼠海绵体神经进行钳夹(但不剪断)以造成海绵体神经损伤所致勃起功能障碍(erectile dysfunction,ED)大鼠模型;术后5 d采用不同剂量黄芪(分别设置黄芪30、120 g)的补阳还五汤灌胃干预,假手术组与模型组均予以等量生理盐水灌胃,每日1次,连续30 d。30 d后测定大鼠阴茎海绵体内压(intracavernous pressure,ICP),Masson染色技术观察海绵体组织平滑肌与胶原沉积含量,免疫应荧光技术和Western Blot检测各组神经型一氧化氮合酶(neuronal nitric oxide synthase,nNOS),神经丝蛋白轻链多肽(neurofilament light polypeptide,NF-L),生长相关蛋白43(growth associated protein 43,GAP-43)变化。结果与假手术组相比,模型组大鼠ICP明显降低(P<0.01),阴茎海绵体平滑肌面积/胶原面积减少(P<0.01),NF-L、nNOS、GAP43水平明显下降(P<0.01);与模型组相比,30 g组与120 g组大鼠ICP明显增高(P<0.01),阴茎海绵体平滑肌面积/胶原面积增高(P<0.01),nNOS、NF-L、GAP43水平明显增高(P<0.01),其中与30 g组相比,120 g组大鼠ICP明显增高(P<0.05),阴茎海绵体平滑肌面积/胶原面积增高(P<0.05),nNOS、NF-L、GAP43水平明显增高(P<0.01)。结论补阳还五汤可以明显改善大鼠勃起功能障碍,且《医林改错》原方所载的补阳还五汤治疗效果更优。Objective To explore the therapeutic effects of two different doses of Buyang Huanwu decoction containing Astragalus on erectile dysfunction(ED)in rats with cavernous nerve injury.Methods Thirty-two Sprague-Dawley(SD)rats were randomly divided into four groups:sham operation model,30 g(model+Buyang Huanwu decoction containing 30 g Astragalus)and 120 g(model+Buyang Huanwu decoction containing 120 g Astragalus).The cavernous nerve of the rats was surgically clamped(but not cut)to establish the rat model of ED caused by cavernous nerve injury.Five days after the operation,a 30 g or 120 g dose of Astragalus was used for the gavage intervention of Buyang Huanwu decoction,or the same volume of normal saline(sham and model groups)was given,once a day for 30 consecutive days.After 30 days,the intracavernous pressure(ICP)of the rat corpus cavernosum was measured.Masson staining was used to observe the content of smooth muscle and collagen deposition in the spongy tissue.Fluorescence and western blotting were used to detect changes in neuronal nitric oxide synthase(nNOS),neurofilament light polypeptide(NF-L),growth associated protein 43(GAP-43)expression in each group.Results Compared with the sham group,the model group showed significantly decreased ICP(P<0.01),decreased area of penile smooth muscle/collagen(P<0.01),and significantly decreased levels of NF-L,nNOS and GAP43(P<0.01).Compared with the model group,the rats in the 30 g and 120 g Astragalus groups showed significantly increased ICP(P<0.01),increased area of penile smooth muscle/collagen(P<0.01),and significantly increased levels of nNOS,NF-L and GAP43(P<0.01).Compared with the 30 g group,the rats in the 120 g Astragalus group showed significantly increased ICP(P<0.05),increased area of penile smooth muscle/collagen(P<0.05),and significantly increased levels of nNOS,NF-L and GAP43(P<0.01).Conclusions Buyang Huanwu decoction containing Astragalus obviously improved ED in cavernous nerve injury model rats.The higher dose of Astragalus(120 g),which is used in the o

关 键 词：补阳还五汤 海绵体神经损伤 勃起功能障碍 黄芪 海绵体内压 

分 类 号：Q95-33[生物学—动物学]