微型CT在血管钙化动物模型中的应用进展  

Advances in the application of micro-CT in animal models of vascular calcification

作　　者：王泽静[1] 王询 肖康[1] 张晶[2] WANG Zejing;WANG Xun;XIAO Kang;ZHANG Jing(Department of CT Room,the First Hospital of Qinhuangdao,Qinhuangdao 066000,China;Department of Cardiology,the First Hospital of Qinhuangdao,Qinhuangdao 066000)

机构地区：[1]河北省秦皇岛市第一医院CT室,河北秦皇岛066000 [2]河北省秦皇岛市第一医院心内科,河北秦皇岛066000

出　　处：《中国实验动物学报》2020年第6期853-856,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金课题(30871086);秦皇岛市科技计划(201602A239);秦皇岛市科学技术研究与发展计划(201902A180)。

摘　　要：血管钙化是钙盐过度沉积于血管壁导致的异位钙化现象,是动脉粥样硬化的病理表现,是糖尿病、高血压病、冠状动脉粥样硬化性心脏病、脑梗死、肾功能衰竭等多种疾病的共有的病理生理过程。目前的CT技术可以完整的重建钙化的动脉并提供准确的量化信息。高分辨率的微型CT可以区分和量化宏观和微观的血管钙化。将CT与18F-NaF micro-PET/CT相结合,可以增强体内活动的微钙化的检测,并帮助我们了解不同信号通路和药物在血管钙化发展中的作用。更重要的是3D微型CT可以和组织病理学、免疫组化、蛋白组学处理相结合,提供同一血管片段的互补信息。微型CT的技术进步可以为血管钙化的动物活体研究提供更多的方法。Vascular calcification is an ectopic calcification phenomenon caused by excessive deposition of calcium salts in the vascular wall.This pathological manifestation is common in diseases such as atherosclerosis,diabetes,hypertension,and chronic renal insufficiency;it is an important risk factor for cardiovascular and cerebrovascular diseases.Current computed tomography(CT)techniques can completely reconstruct calcified arteries and provide accurate quantitative information.High resolution micro-CT can distinguish and quantify areas of macroscopic and microscopic vascular calcification.CT combined with 18F-sodium Flfluoride(18F-NaF)micro-PET/CT can enhance the detection of microcalcification in vivo and aid in understanding the roles of signaling pathways and drugs in the development of vascular calcification.Furthermore,three-dimensional micro-CT can be combined with histopathological,immunohistochemical,and proteomic analysis method to provide complementary information regarding a single vascular segment.Advances in micro-CT techniques may provide additional method for the analysis of vascular calcification in animals in vivo.

关 键 词：血管钙化 微型CT 动物模型 

分 类 号：Q95-33[生物学—动物学]