海南狗牙花有效部位提取和体外抗肿瘤活性研究  

Study on the Extraction of Active Parts from rvatamia hainanensis and Its Anti-tumor Activity in vitro

作　　者：潘显茂 郑英换 李兰婷 孔海洁 吴佳颖 PAN Xian-mao;ZHENG Ying-huan;LI Lan-ting(Hainan Institute of Pharmacology,Haikou,Hainan 570311)

机构地区：[1]海南省药物研究所,海南海口570311 [2]海南广药晨菲医药有限公司,海南海口570208

出　　处：《安徽农业科学》2021年第1期160-161,171,共3页Journal of Anhui Agricultural Sciences

基　　金：海南省科研专项(KYYS-2018-39)。

摘　　要：[目的]初步筛查海南狗牙花抗肿瘤活性部位。[方法]采用MTT比色法测定海南狗牙花4种提取物分别对人非小细胞肺癌细胞(A549)和人乳腺癌细胞(MCF-7)体外生长抑制作用。[结果]海南狗牙花4种提取物对2种肿瘤细胞呈现不同的抑制活性,乙酸乙酯提取物的抗肿瘤活性最强,石油醚提取物次之,正丁醇萃取物较弱而萃取后的水层无抗肿瘤活性。[结论]该研究为深入研究海南狗牙花中具有抗肿瘤活性的单体化合物提供科学依据。[Objective]To initially screen the anti-tumor active parts of Ervatamia hainanensis.[Method]MTT colourometry was used to determine the inhibitory effect of four extracts of Ervatamia hainanensis on A549 and MCF-7 human tumor cells in vitro.[Result]The four extracts of Ervatamia hainanensis showed different inhibitory activities on two kinds of tumor cells,the anti-tumor activity of the ethyl acetate extract was the strongest,the petroleum ether was the second,the n-butanol extract was weak,and the aqueous layer after the extraction had no anti-tumor activity.[Conclusion]This research provides scientific basis for the in-depth study of the monomeric compounds with anti-tumor activity in Ervatamia hainanensis.

关 键 词：海南狗牙花 提取物 抗肿瘤活性 MTT比色法 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]