促红细胞生成素预处理增强大鼠骨髓间充质干细胞定向归巢能力的初步研究  

Preliminary study of effect of erythropoietin pretreatment on enhancing directional homing ability of bone marrow mesenchymal stem cells in rats

作　　者：乔禹铭 周松 张亚 刘永光[1] 赵明[1] Qiao Yuming;Zhou Song;Zhang Ya;Liu Yongguang;Zhao Ming(Department of Organ Transplantation,Zhujiang Hospital of Southern Medical University,Guangzhou 510280,China)

机构地区：[1]南方医科大学珠江医院器官移植科,广州510280

出　　处：《器官移植》2021年第1期57-63,共7页Organ Transplantation

基　　金：广东省科技计划项目(2016A020220014)。

摘　　要：目的探讨促红细胞生成素(EPO)对大鼠骨髓间充质干细胞(BMSC)增殖及迁移能力的影响。方法将第5代BMSC分为5组,分别为对照组(不加EPO)及10、100、500、1000 IU/mL EPO组,培养24 h和48 h后检测各组BMSC的增殖率、迁移能力以及趋化因子受体(CXCR)4的表达情况。将第5代BMSC分为BMSC组和EPO-BMSC组,培养48 h后观察EPO对两组BMSC的表面标志物、定向分化和细胞骨架形态的影响。结果EPO与BMSC共培养48 h后,与对照组比较,100、500 IU/mL EPO组BMSC增殖率和迁移能力增强,CXCR4蛋白表达量增高(均为P<0.05)。EPO-BMSC组BMSC表面标志物的表达和定向分化能力未受EPO影响。在EPO-BMSC组中,大多数细胞的纤维骨架沿细胞的长轴排列,呈平行状。结论EPO可提高BMSC的增殖率、迁移能力和组织修复治疗能力,可能通过增加CXCR4的表达,促进其向损伤器官组织的定向归巢。Objective To evaluate the effect of erythropoietin(EPO)on the proliferation and migration of bone marrow mesenchymal stem cell(BMSC)in rats.Methods The 5th generation BMSCs were divided into the control(without EPO)and 10,100,500,1000 IU/mL EPO groups.After 24 h and 48 h of culture,the proliferation rate,migration ability and the expression levels of CXCR4 of BMSCs were detected in each group.The 5th generation BMSCs were further divided into BMSC and EPO-BMSC groups.After 48 h of culture,the effect of EPO upon surface markers,directional differentiation and cytoskeleton morphology of BMSCs were evaluated in both groups.Results After the co-culture of EPO and BMSCs for 48 h,the proliferation rate and migration ability of BMSCs were significantly enhanced,and the expression level of CXCR4 protein was significantly up-regulated in the 100 IU/mL and 500 IU/mL EPO groups compared with those in the control group(all P<0.05).However,EPO exerted no effect upon the expression levels of surface markers and directional differentiation ability of BMSCs in the EPO-BMSC group.In the EPO-BMSC group,the fibrous skeleton of most BMSCs was arranged along the long axis in parallel.Conclusions EPO can improve the proliferation rate,migration ability and tissue repair capability of BMSCs,probably by promoting the directional homing of BMSCs to injured organs and tissues via up-regulating the expression level of CXCR4.

关 键 词：骨髓间充质干细胞 移植 促红细胞生成素 归巢 迁移 增殖 趋化因子受体 分化 

分 类 号：R617[医药卫生—外科学]