基于ISSR的地黄栽培品种与野生群体遗传多样性研究  

Genetic diversity analysis on cultivar and wild population of Rehmannia glutinosa based on ISSR markers

作　　者：李翠翠 胡赛文 夏至[1] LI Cui-cui;HU Sai-wen;XIA Zhi(College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学农学院,河南郑州450002

出　　处：《中草药》2020年第23期6054-6061,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(31770370);河南省高等学校重点科研项目计划(18A360006)。

摘　　要：目的基于ISSR分子标记研究地黄Rehmannia glutinosa栽培品种与野生群体遗传多样性,比较它们的遗传多样性差异,构建它们之间亲缘关系,为地黄种质资源保护及新品种选育提供参考。方法应用ISSR分子标记技术对地黄栽培品种,野生群体及其近缘种106个样品进行分析,利用POPGEN软件分析Nei’s基因多样性指数(H)等遗传信息参数,应用NTSYS软件构建亲缘关系UPGMA聚类图。结果 7条引物在所有取样群体中,共检测到85个位点,其中多态性位点83个,多态位点百分率(PPB)为97.65%。地黄栽培品种与野生群体及其近缘种的种群间H值为0.265 9,Shannon’s多样性信息指数(I)为0.412 5。地黄栽培品种的多态性位点26个,PPB为30.59%。河南省地黄野生群体多态性位点71个,PPB为83.53%。ISSR聚类分析结果显示地黄栽培品种,野生群体及其近缘种共分成7个组。结论 ISSR分子标记揭示地黄野生群体比栽培品种具有更高的遗传多样性,河南分布的野生地黄群体的遗传多性高于其他地区,与其作为栽培地黄的道地产区,具有一定的相关性。地黄野生群体间亲缘关系与其地理分布格局并无明显相关性。Objective To study the genetic diversity and genetic relationship of cultivar and wild population of Rehmannia glutinosa and its relative species by ISSR molecular marker technique, and provide the reference for R. glutinosa germplasm protection and breeding. Methods 106 samples including cultivar and wild population of R. glutinosa and its relative species were studied by ISSR-PCR markers. Nei’s genetic diversity index(H) and other parameters of genetic information were calculated by POPGEN 32, and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean(UPGMA) by NTSYS-pc software. Results Seven ISSR primers generated 85 loci of which 83 loci were polymorphic. The percentage of polymorphie bands(PPB) of all samples was 97.65%. Nei’s genetic diversity index(H) and Shannon’s information index(I) were 0.2659 and 0.4125. The percentage of polymorphie bands(PPB) of cultivar of R. glutinosa was 30.59%. The percentage of polymorphie bands(PPB) of wild population of R.glutinosa in Henan province was 83.53%. In the cluster dendrogram, all samples were clustered into seven groups at the level of Genetic similarity coefficient(GS) 0.67. Conclusion The results of ISSR analysis revealed that the level of genetic diversity between wild populations of R. glutinosa was higher than that within cultivar populations of R. glutinosa. The genetic diversity among wild populations of R. glutinosa in Henan province was higher than other region, which was consistent with authentic producing areas of R. glutinosa in this area. The relationships of wild population of R. glutinosa had no obvious correlation with their geographical distribution pattern.

关 键 词：地黄 栽培 野生群体 ISSR 遗传多样性 

分 类 号：R286.12[医药卫生—中药学]