Phenylpropanoid Derivatives Are Essential Components of Sporopollenin in Vascular Plants  

作　　者：Jinp-Shi Xue Baocai Zhang HuaDong Zhan Yong-Lin Lv Xin-Lei Jia TianHua Wang Nai-Ying Yang Yu-Xia Lou Zai-Bao Zhang Wen-Jing Hu Jinshan Gui Jianguo Cao Ping Xu Yihua Zhou Jin-Feng Hu Laigeng Li Zhong-Nan Yang 

机构地区：[1]Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China [2]National Key Laboratory of Plant Molecular Genetics&CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Beijing 200032,China [3]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [4]State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China [5]College of Life Science,Xinyang Normal University,Xinyang,Henan 464000,China [6]Department of Natural Products Chemistry,School of Pharmacy,Fudan University,No.826 Zhangheng Road,Shanghai,201203,China

出　　处：《Molecular Plant》2020年第11期1644-1653,共10页分子植物（英文版）

基　　金：grants from the National Key Research and Development Program of China(2016YFD0100902);the National Natural Science Foundation of China(31900165,31870296,31700277);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB27020104);the Program from Shanghai Municipal Education Com mission[2019-01-07-00-02-E00006];the China Postdoctoral Science Foundation(2017M61159);Jiangsu Collaborative Innovation Center for Modern Crop Production(to H.D.Z.).

摘　　要：The outer wall of pollen and spores,namely the exine,is composed of sporopollenin,which is highly resistant to chemical reagents and enzymes.In this study,we demonstrated that phenylpropanoid pathway derivatives are essential components of sporopollenin in seed plants.Spectral analyses showed that the autofluorescence of Lilium and Arabidopsis sporopollenin is similar to that of lignin.Thioacidolysis and NMR analyses of pollen from Lilium and Cryptomeria further revealed that the sporopollenin of seed plants contains phenylpropanoid derivatives,including p-hydroxybenzoate(p-BA),p-coumarate(p-CA),ferulate(FA),and lignin guaiacyl(G)units.The phenylpropanoid pathway is expressed in the tapetum in Arabidopsis,consistent with the fact that the sporopollenin precursor originates from the tapetum.Further germination and comet assays showed that this pathway plays an important role in protection of pollen against UV radiation.In the pteridophyte plant species Ophioglossum vulgatum and Lycopodium clavata,phenylpropanoid derivatives including p-BA and p-CA were also detected,but G units were not.Taken together,our results indicate that phenylpropanoid derivatives are essential for sporopollenin synthesis in vascular plants.In addition,sporopollenin autofluorescence spectra of bryophytes,such as Physcomitrella and Haplocladium,exhibit distinct characteristics compared with those of vascular plants,indicating the diversity of sporopollenin among land plants.

关 键 词：SPOROPOLLENIN pollen cell wall phenylpropanoid pathway 

分 类 号：Q94[生物学—植物学]