核型自动扫描系统在淋巴细胞染色体畸变检测中的价值  

The value of karyotype automatic scanning system in the detection of lymphocyte chromosome aberrations

作　　者：钱小莲 方慧莲 QIAN Xiaolian;FANG Huilian(Nanjing Prevention and Treatment Center for Occupational Diseases,Nangjing 210042 China)

机构地区：[1]南京市职业病防治院检验科,江苏南京210042

出　　处：《中国辐射卫生》2020年第5期470-472,共3页Chinese Journal of Radiological Health

摘　　要：目的探讨核型自动扫描成像系统在淋巴细胞染色体畸变检测中的优点。方法以192名医疗单位的放射工作人员为研究对象,分别用核型自动扫描系统检测法和人工双盲检测法对其外周血淋巴细胞染色体畸变细胞率和染色体畸变率进行检测分析。结果对同一批标本阅片,扫描组平均用时(15.3±0.69)min小于人工组(42.0±0.56)min,差异有统计学意义(P<0.05)。对同一批标本,扫描组的染色体畸变细胞率(0.193%)和染色体畸变率(0.227%)均高于人工组(0.065%,0.102%,),差异均有统计学意义(P<0.05)。结论核型自动扫描系统可以提高淋巴细胞染色体畸变的检出率,提高阅片的速度,缩短发报告的周期,减轻实验室人员的工作量,适合标本量较多的单位使用。Objective The study is to investigate the value of karyotype automatic scanning system in the detection of lymphocyte chromosome aberrations.Methods 192 radiation workers in medical units were selected and measured the rate of chromosome aberrations and chromosome aberration cells by two ways which are karyotype automatic scanning system and manual double blind experiment.Results Reading the same batch of specimens,the average time(15.3±0.69,min)of scanning system is shorter than that(42.0±0.56,min)of manual double blind way,there were statistically significant differences(P<0.05).Using scanning system,the rate(0.193%)of chromosome aberrations and(0.227%)chromosome aberration cells were higher than those(0.065%,0.102%)with manual double blind experiment which was statistically significant differences(P<0.05).Conclusion The karyotype automatic scanning system can improve the detection rate of chromosome aberrations and the reading speed.Moreover,it can shorten the reporting period and reduce the workload of laboratory personnel.It is suitable for units with more specimens.

关 键 词：核型自动扫描系统 染色体畸变细胞率 染色体畸变率 

分 类 号：R816.3[医药卫生—放射医学]