筇竹组培快繁技术  

Technical Research on Rapid Propagation of Qiongzhuea tumidinoda Tissue Culture

作　　者：郑静楠 董文渊[2] 钟欢 尹泽南 吴义远 Zheng Jingnan;Dong Wenyuan;Zhong Huan;Yin Zenan;Wu Yiyuan(Yunnan Forestry Investigation and Planning Institute,Kunming 650000,P.R.China;Southwest Forestry University)

机构地区：[1]云南省林业调查规划院,昆明650051 [2]西南林业大学

出　　处：《东北林业大学学报》2021年第1期50-54,59,共6页Journal of Northeast Forestry University

基　　金：国家林业公益性行业科研专项(201204103);中央财政林业科技推广示范资金项目([2018]TG14号)。

摘　　要：以筇竹种胚为外植体,MS为基本培养基,通过无菌体系的建立、丛芽诱导增殖、生根培养研究其组培技术,筛选移栽介质。结果表明:在组培无菌条件下,采用体积分数0.5%甲醛(12 h)→体积分数75%酒精(15 min)→体积分数0.1%升汞(8 min)的处理效果较为理想,筇竹种胚污染率为3.33%,萌芽率为86.21%,成活率为83.33%;筛选出MS+2.0 mg·L^-16-BA+0.2 mg·L^-1NAA适合筇竹种胚的丛芽诱导;1/2MS+0.3 mg·L^-1NAA+1.0 mg·L^-1KT+1.0 mg·L^-16-BA适合筇竹分化丛芽增殖;1/2MS+0.3 mg·L^-16-BA+2.0mg·L^-1NAA+0.1 mg·L^-1IBA适合筇竹的生根培养;m(河沙)∶m(珍珠岩)∶m(腐殖土)=1∶1∶2的基质为最佳移栽质量配比。The embryos of Qiongzhuea tumidinoda were used as explants, MS was the basic medium, and the tissue culture technique was studied through the establishment of sterile system, bud-induced proliferation and rooting culture, and the transplanted soil was screened. The results showed that under the aseptic conditions of tissue culture, the treatment with 0.5% formaldehyde(12 h)→75% alcohol(15 min)→0.1% mercuric chloride(8 min) was ideal, and the contamination rate of Q. tumidinoda seed embryo was 3.33%. The survival rate was 86.21%, and the survival rate was 83.33%. MS+6-BA2.0 mg·L^-1+NAA0.2 mg·L^-1 was selected for the induction of shoots of Q. tumidinoda seed embryos;1/2 MS+NAA0.3 mg·L^-1+KT1. 0 mg·L^-1+ 6-BA1.0 mg·L^-1 is suitable for bamboo differentiation bud proliferation;1/2 MS+6-BA0.3 mg·L^-1+NAA2 mg·L^-1+IBA0.1 mg·L^-1 is suitable rooting of Q. tumidinoda;Hesha∶Perlite∶The 1∶1∶2 substrate of humus is the best transplant ratio.

关 键 词：筇竹 种胚 组培 移栽 

分 类 号：S795[农业科学—林木遗传育种]