3-硝基丙酸诱导小鼠睾丸氧化应激模型的建立  

Establishment of mouse testicular oxidative stress model induced by 3-nitropropionic acid

作　　者：王慧瑞 贺海旺 于佳彤 郭庸 赵晨序 柳海星[1] WANG Huirui;HE Haiwang;YU Jiatong;GUO Yong;ZHAO Chenxu;LIU Haixing(Agricultural College of Yanbian University,Yanji Jilin 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133000

出　　处：《延边大学农学学报》2020年第4期62-66,共5页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金项目(31360546)。

摘　　要：大量研究已表明,氧化应激能够引起多种睾丸相关疾病,严重危害人类和动物的生殖健康。为建立小鼠睾丸的氧化应激模型,对小鼠腹腔注射不同浓度的3-硝基丙酸(3-NPA)。试验随机选取20只昆明雄性小鼠分成对照组与3-NPA注射组。3-NPA组中设立3个浓度梯度,分别为低浓度组(6.25 mg/kg)、中浓度组(12.5 mg/kg)、高浓度组(25 mg/kg)。模型组对腹腔注射各浓度计量3-NPA,对照组注射生理盐水早晚2次,中间间隔12 h,共注射7 d,于最后1次腹腔注射后对小鼠进行公母合笼,等候观察并记录其见栓率,然后继续跟进记录其产子数。结果表明:3-NPA可成功诱导建立小鼠睾丸氧化应激模型,且其诱导氧化应激模型的最适浓度为12.5 mg/kg。In order to establish the oxidative stress model of mouse testis,different concentrations of 3-NPA were injected intraperitoneally.Randomly,20 Kunming male mice were selected and divided into experimental and control groups.Experimental groups were injected with 3-NPA intraperitoneally in three different concentrations(6.25 mg/kg,12.5 mg/kg and 25 mg/kg).In contrast,the control group was injected with saline.The injection process was executed twice in the morning and evening,with an interval of 12h for a total of 7 days.After the final injection,the mice were caged in gender couples for observation of thrombosis and birth rate.The results showed that 3-NPA at optimal concentration of 12.5mg/kg can be considered one of the potent agents for establishment of a mouse testicular oxidative stress model.

关 键 词：睾丸 氧化应激 3-NPA 小鼠 

分 类 号：S852.2[农业科学—基础兽医学]