交叉电项针治疗脑出血后咳嗽反射障碍豚鼠安全性研究  

Safety research on treatment of cough reflex disorder after cerebral hemorrhage with crossing nape electroacupuncture in guinea pig

作　　者：贾坤平 裴思颖 王虹 王鑫 蔡国锋[1,3] JIA Kun-ping;PEI Si-ying;WANG Hong;WANG Xin;CAI Guo-feng(Internal Medicine,Hanan Branch of the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150060,China;Graduate School of Heilongjiang University of Chinese Medicine,Harbin 150040;Department of Acupuncture,Heilongjiang Traditional Chinese Medicine Hospital,Harbin 150001)

机构地区：[1]黑龙江中医药大学附属第二医院哈南分院内科,哈尔滨150060 [2]黑龙江中医药大学研究生院,哈尔滨150040 [3]黑龙江省中医医院针灸科,哈尔滨150001

出　　处：《针刺研究》2020年第12期954-960,共7页Acupuncture Research

基　　金：黑龙江中医药大学科研基金面上项目(No.2019MS19);黑龙江省自然科学基金面上项目(No.H2018066)。

摘　　要：目的:观察交叉电项针对脑出血后咳嗽反射障碍豚鼠咳嗽反射次数(CCnt)、咳嗽潜伏期和血常规、肝功能、肾功能、心肌酶及心、肝、肾脏组织病理结构的影响,评价交叉电项针治疗脑出血后咳嗽反射障碍的有效性和安全性。方法:雄性纯白豚鼠随机分为空白组、模型组、针刺组、交叉电项针组和电项针组,每组又分为术后1、3、7、14 d 4个亚组,每个亚组各8只。采用自体血注入法复制豚鼠脑出血后咳嗽反射障碍模型。针刺组针刺双侧"风池""翳风";交叉电项针组在针刺组基础上电针正极连接"翳风",负极连接对侧"风池";电项针组在针刺组基础上电针正极连接"翳风",负极连接同侧"风池"。电针频率2 Hz,强度0.5 mA,各组治疗每次均为20 min,每日1次。采用Longa评分法对脑出血后豚鼠神经功能进行评估;分析Buxco装置记录的CCnt和咳嗽潜伏期变化;采用全自动血液分析仪检测豚鼠血常规,全自动生化分析仪检测肝功能、肾功能、心肌酶等实验室指标变化;采用透射电镜观察各组豚鼠心脏、肝脏、肾脏组织病理结构变化。结果:与空白组比较,模型组豚鼠各时段神经功能缺损评分均显著升高(P<0.01);与模型组比较,针刺组、交叉电项针组、电项针组神经功能缺损评分均降低(P<0.01,P<0.05)。与空白组比较,模型组各时段CCnt均显著减少(P<0.01),咳嗽潜伏期均显著延长(P<0.01);与模型组比较,3个治疗组术后7 d和14 d CCnt均增多(P<0.05,P<0.01),咳嗽潜伏期均缩短(P<0.05,P<0.01);7 d和14 d时,交叉电项针组较其他两治疗组CCnt明显增多(P<0.05,P<0.01),咳嗽潜伏期明显缩短(P<0.05,P<0.01);电项针组14 d时这两个指标的改善程度优于针刺组(P<0.05)。与空白组比较,模型组白细胞(WBC)计数在术后3、7、14 d均明显升高(P<0.01);与模型组比较,术后3 d时交叉电项针组WBC计数降低(P<0.05),术后7 d和14 d时3个治疗组WBC计数均降低(P<0.01,PObjective To observe the effect of crossing nape electroacupuncture(EA)on cough count(CCnt),cough incubation period and blood routine,liver function,renal function,myocardial enzymology,and heart,liver and kidney pathological structure in guinea pig with cough reflex disorder after cerebral hemorrhage,so as to evaluate the efficacy and safety of crossing nape EA in the treatment of cough reflex disorder after cerebral hemorrhage.Methods A total of 160 male pure white guinea pigs were randomly divided into normal group,model group,acupuncture group,crossing nape EA group and nape EA group(n=32 in each group).These 5 groups were further divided into 1,3,7 and 14 d subgroups(n=8 in each subgroup).The model of cough reflex disorder after cerebral hemorrhage was established by injection of the guinea pigs’autoblood(50μL)into the basal ganglia region confirmed by Longa’s neurologic examination grading system(0—4 points).Acupuncture was applied to bilateral"Fengchi"(GB20)and"Yifeng"(SJ17)in the acupuncture group.In the crossing nape EA group,the positive pole of the needle is connected to SJ17,while the negative pole is connected to the opposite side of GB20(2 Hz,0.5 mA).In the nape EA group,the positive pole of the needle is connected to SJ17,while the negative pole is connected to the same side of GB20(2 Hz,0.5 mA).All treatments were applied 20 min each time,once a day.The changes of CCnt and cough incubation period were recorded by Buxco device.The changes of blood routine,liver function,kidney function,myocardial enzyme and other laboratory indexes were monitored by automatic analyzer instrument.The pathological changes of heart,liver and kidney of guinea pigs were observed by transmission electron microscopy.Results Compared with the normal group,the guinea pigs’neurological deficit scores in the model group were significantly increased at each time point(P<0.01).Compared with the model group,the neurological deficit scores of the acupuncture group,crossing nape EA group and nape EA group at 3 d,7 d and 1

关 键 词：交叉电项针 脑出血 咳嗽反射 安全性 

分 类 号：R245.97[医药卫生—针灸推拿学]