Genkwadane F-I,New Daphnane-type Diterpenes from the Flower Buds of Daphne genkwa Sieb.et Zucc.Exhibit Anti-tumor Activities via lnducing Apoptosis  

作　　者：Xuewen Hou Jingjie Chen Xuefen Hou Pinyi Gao Jinlin Wang Shaojiang Song Lingzhi Li 

机构地区：[1]School of Traditional Chinese Materia Medico,Key Laboratory of Structure-Based Drug Design and Discovery,Ministry of Education,Shenyang Pharmaceutical University,103 Wenhua Road,Shenyang,Liaoning 110016,China [2]College o f Pharmaceutical and Biotechnology Engineering,Institute of Functional Molecules,Shenyang University of Chemical Technology,11 Street,Shenyang Economic and Technological Development Zone,Shenyang,Liaoning 110142,China

出　　处：《Chinese Journal of Chemistry》2020年第12期1600-1606,共7页中国化学（英文版）

基　　金：This research was supported by the National Nature Science Foundation of China(Nos.81872766,81673324,81573319);the Foundation(L2015518)from the Project of Education Departmentof Liaoning Province of China,the Project of Innovation Team(LT2015027);of Liaoning and the Liaoning province Natural Science Foundation of China(201602705).

摘　　要：of main observation and conclusion Genkwadane F-I(1-4),four new diterpenes,along with four known ones(5-8),were isolated from the flower buds of D.genkwa.The structures including absolute configurations of 1-4 were determined based on the analysis of NMR and ECD calculations.All compounds were evaluated for their cytotoxicities against MCF-7 and Hep3B cell lines,among which compounds 3 and 4 showed significant activities.Compound 4 exhibited the most potent activity with IC50 values of 1.33μM against MCF-7,and the IC50 values of compound 3 against MCF-7 and Hep3B were 5.54μM and 8.73μM;respectively.Further studies by Hoechst 33258 staining and Annexin V/PI analysis demonstrated that compounds 3 and 4 could induce apoptosis to exhibit anti-tumor activities.

关 键 词：absolute CONCLUSION compounds 

分 类 号：O62[理学—有机化学]