全自动核酸检测分析系统在无偿献血者病毒检测中的应用  

Role of automatic nucleic acid detection analysis system in detecting of virus in voluntary blood donors

作　　者：刘永霞[1] 刘欢[1] 刘燕[1] 赵妮 LIU Yongxia;LIU Huan;LIU Yan;ZHAO Ni(Department of Blood Transfusion,Affiliated Hospital of Yan′an University,Yan′an,Shaanxi,716000)

机构地区：[1]延安大学附属医院输血科,陕西延安716000

出　　处：《实用临床医药杂志》2020年第23期113-115,共3页Journal of Clinical Medicine in Practice

摘　　要：目的观察全自动核酸检测分析系统在无偿献血者血液乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)和人类免疫缺陷病毒(HIV)检测中的应用效果。方法选取无偿献血者1229例作为研究对象,每位献血者采集2管静脉血,1管用于酶联免疫吸附试验(ELISA)检测,另1管用于核酸检测。结果核酸检测、ELISA检测的HBV阳性率分别为2.03%、0.98%,差异有统计学意义(P<0.05);核酸检测、ELISA检测的HCV阳性率分别为1.22%、1.14%,差异无统计学意义(P>0.05);核酸检测、ELISA检测的HIV阳性率分别为0.24%、1.06%,差异有统计学意义(P<0.05)。结论全自动核酸检测分析系统应用于无偿献血者血液HBV、HCV和HIV检测中具有较高的筛查准确率,与ELISA检测方法比较各具优势,2种方法联合应用可提高病毒检测的准确性。Objective To observe the effect of automatic nucleic acid detection analysis system in detection of hepatitis B virus(HBV),hepatitis C virus(HCV)and human immunodeficiency virus(HIV)in blood samples of blood donors.Methods A total of 1229 volunteer blood donors were selected,two copies of blood in each donor were collected,1 copy was used for Enzyme Linked Immunosorbent Assay(ELISA)and another one was used for nucleic acid detection.Results The positive ratesof HBV by nucleic acid test and ELISA test were 2.03%and 0.98%,respectively,there was significant difference(P<0.05).The positive rates of nucleic acid test and ELISA in detecting HCV were 1.22%and 1.14%,there was no significant difference(P>0.05).The positive rates of nucleic acid test and ELISA in HIV detection were 0.24%and 1.06%,there was significant difference(P<0.05).Conclusion Automatic nucleic acid detection analysis system has higher accuracy rate in screening HBV,HCV and HIV in voluntary blood donors,nucleic acid detection and ELISA detection have their own advantages,and the combination of the two methods can improve the accuracy of virus detection.

关 键 词：全自动核酸检测 无偿献血者 乙型肝炎病毒 丙型肝炎病毒 人类免疫缺陷病毒 

分 类 号：R446.6[医药卫生—诊断学] R512.6[医药卫生—临床医学]