猪血浆球蛋白的高效制备研究  

On Efficient Preparation of Porcine Plasma Globulin

作　　者：杨海[1] 张淇美 龚慧芳 唐青海[1] 阳希 岳跃飞 Yang Hai;Zhang Qimei;Gong Huifang;Tang Qinghai;Yang Xi;Yue Yuefei(Key Laboratory of Conservation and Utilization of Biological Resources in Nanyue Mountain Area,Hengyang Normal University,Hengyang Hunan 421008,China;Research Center of Hunan Blood Products Engineering Technology,Nanyue Biopharmaceutical Co.,Ltd..,Hengyang Hunan 421007,China)

机构地区：[1]衡阳师范学院南岳山区生物资源保护与利用湖南省重点实验室,湖南衡阳421008 [2]南岳生物制药有限公司湖南省血液制品工程技术研究中心,湖南衡阳421007

出　　处：《衡阳师范学院学报》2020年第6期130-134,共5页Journal of Hengyang Normal University

基　　金：湖南省自然科学基金-衡阳联合基金项目(2018JJ4023);国家级大学生创新创业训练计划项目(CX1801)。

摘　　要：为探索猪血浆球蛋白的高效制备工艺,试验以新鲜猪血为原料,差速离心法获得猪血浆,探明血浆稀释倍数、pH、乙醇浓度和温度四个影响球蛋白制备的关键参数,通过三次乙醇反应和纯化操作,从猪浆血中提取免疫球蛋白。结果表明:猪血浆球蛋白适宜的制备条件为:第一次乙醇反应:pH 7.0;第二次乙醇反应:蛋白浓度稀释12倍、pH 5.5、乙醇浓度15%;第三次乙醇反应:pH 7.0、乙醇浓度18%;制备过程温度为-4℃至-5℃,在此条件下的猪血浆球蛋白回收率和纯度可以分别达到30%和99%。此制备工艺为规模化制备猪血浆球蛋白提供了重要参考。In order to explore the efficient preparation technology of pig plasma globulin,pig plasma was obtained by differential centrifugation from fresh pig blood.The four key parameters of plasma dilution ratio,pH,ethanol concentration and temperature were explored,and then pig plasma globulin was extracted from pig plasma through three ethanol reactions and purification operations.The results showed that the optimal conditions for the preparation of pig plasma globulin were as follows:the first ethanol reaction:pH 7.0;the second ethanol reaction:protein dilution 12 times,pH 5.5;ethanol concentration 15%;the third ethanol reaction:pH 7.0,ethanol concentration 18%;the whole operation temperature was-4℃to-5℃.Under these conditions,the recovery rate and purity of pig plasma globulin could reach 30%and 99%,respectively.This preparation process provides an important reference for large-scale preparation of pig plasma globulin.

关 键 词：猪血浆 球蛋白 高效制备 乙醇反应 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]