促凋亡基因7对急性髓系白血病细胞的增殖、凋亡的影响  

Effect of PDCD7 on the Proliferation and Apoptosis of Kasumi-1 Cells

作　　者：黄走方[1] 廖长风[1] 严志民[1] 陈懿建[1] HUANG Zou-fang;LIAO Chang-feng;YAN Zhi-min;CHEN Yi-jian(Department of Hematology,First Affiliated Hospital of Gannan Medical University,Jiangxi Ganzhou 341000,China)

机构地区：[1]赣南医学院第一附属医院血液科,江西赣州341000

出　　处：《循证医学》2020年第3期186-188,192,共4页The Journal of Evidence-Based Medicine

基　　金：江西省卫生厅科技计划(20121093)。

摘　　要：目的探讨促凋亡基因7(PDCD7)对急性髓系白血病(AML)细胞株Kasumi-1细胞增殖、凋亡的影响。方法用针对PDCD7基因的小干扰RNA转染Kasumi-1细胞,下调PDCD7基因表达。用MTT法及台盼蓝拒染细胞技术检测细胞增殖;瑞氏-吉姆萨染色观察细胞形态;PI染色流式细胞术分析细胞周期;Annexin V/PI双染色细胞术及Hoechst染色检测细胞凋亡;Western blot法检测凋亡相关蛋白NF-κB、bcl-2和caspase-3的表达。结果PDCD7-siRNA转染Kasumi-1细胞后,其PDCD7 mRNA表达下调69%,培养后Kasumi-1细胞的增殖、分化、凋亡、细胞周期无明显变化(P>0.05)。结论干扰PDCD7基因表达没有影响到Kasumi-1细胞的增殖、凋亡。Objective To investigate the effect of programmed cell death 7(PDCD7)gene on the proliferation and apoptosis of human acute myeloid leukemia Kasumi-1 cells.Methods We transfected Kasumi-1 cells to down regulate the expression of PDCD7 gene with small interfering RNA.Cell proliferation was detected by MTT assay and trypan.Cell morphology was observed by Wright Giemsa staining.Cell cycle was analyzed by flow cytometry with PI staining.Apoptosis was detected by annexin V/PI double staining and Hoechst staining.Apoptosis related proteins NF-κB,bcl-2 and caspase-3 were detected by Western blot.Results The expression of PDCD7 mRNA in Kasumi-1 cells was down regulated by 69%after transfection of PDCD7 siRNA.The proliferation,differentiation,apoptosis and cell cycle of Kasumi-1 cells did not change significantly(P>0.05).Conclusion The proliferation and apoptosis of Kasumi-1 cells were not affected by interference of PDCD7 gene.

关 键 词：PDCD7 RNA干扰 急性白血病 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]