无比山药丸对氢化可的松致肾阳虚模型小鼠性功能的影响  

Effect of Wubi Shanyao pills on sexual function in mice with kidney-yang-deficiency induced by hydrocortisone

作　　者：陈青青 单超文 苏洁[1] 陈炜[2] 朱加明 陈素红 吕圭源[1] CHEN Qingqing;SHAN Chaowen;SU Jie;CHEN Wei;ZHU Jiaming;CHEN Suhong;LYU Guiyuan(College of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 311400,China;Hangzhou Huqingyutang Pharmaceutical Co.Ltd,Hangzhou 310016,China;Collaborative Innovation Center of Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311400 [2]杭州胡庆余堂药业有限公司,浙江杭州310016 [3]浙江工业大学绿色制药协同创新中心,浙江杭州310014

出　　处：《浙江大学学报（医学版）》2020年第6期697-704,共8页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(81873036);浙江省重点研发计划(2017C03052);浙江省重点实验室项目(2012E10002)。

摘　　要：目的:探讨无比山药丸对氢化可的松致肾阳虚模型小鼠性功能的影响。方法:采用雄性昆明小鼠皮下注射氢化可的松10 d制备肾阳虚模型,灌胃给予无比山药丸(0.91、1.82、2.73 g/kg)连续9周。观察小鼠一般行为学(自主活动、抓力);交配实验检测雄鼠性行为(捕捉、骑跨、射精次数及潜伏期);ELISA法测定血清皮质醇、促肾上腺皮质激素、促黄体生成素、促卵泡生成素、雌二醇、睾酮水平;计算肾脏、附睾、睾丸和精囊器官指数;HE染色观察小鼠睾丸组织形态;TUNEL法检测睾丸细胞凋亡情况;免疫组织化学法检测睾丸组织增殖细胞核抗原(PCNA)蛋白表达水平。结果:无比山药丸能提高肾阳虚模型小鼠自主活动次数、抓力及捕捉次数,缩短捕捉潜伏期(均P<0.01);升高血清睾酮、皮质醇、促肾上腺皮质激素水平,降低血清促卵泡生成素水平(P<0.05或P<0.01);提高睾丸指数(P<0.05),改善睾丸病变;抑制细胞凋亡,促进睾丸PCNA蛋白表达。结论:无比山药丸能改善氢化可的松致肾阳虚小鼠性功能障碍,其机制可能与调节下丘脑-垂体-肾上腺轴,促进睾丸组织细胞增殖,抑制细胞凋亡有关。Objective:To investigate the effect of Chinese medicine Wubi Shanyao pills on sexual function of kidney-yang-deficiency mice induced by hydrocortisone.Methods:Male Kunming mice were injected with hydrocortisone for 10 days to prepare the kidney-yang-deficiency model,and administrated with Wubi Shanyao pills(0.91,1.82,2.73 g/kg)for 9 weeks.The general behaviors of mice(autonomous activity,grasping power)were observed;sexual behaviors(capture,straddle,ejaculation frequency and incubation period)of mice were detected by mating experiment.The serum levels of cortisol,adrenocorticotropic hormone(ACTH),luteinizing hormone(LH),follicle stimulating hormone(FSH),estradiol(E 2)and testosterone were measured by ELIZA.The organ indexes of kidney,epididymis,testis and seminal vesicle were calculated.The histomorphology of testis was observed by HE staining.The apoptosis of testis cells was detected by TUNEL;and the expression level of proliferating cell nuclear antigen(PCNA)in testis was detected by immunohistochemistry.Results:Wubi Shanyao pills increased the number of independent activities,grasping power,capture frequency of model mice and shortened the capture latency(all P<0.01);and also increased the serum levels of testosterone,cortisol,ACTH,decreased the serum levels of FSH(P<0.05 or P<0.01);increased testis index(P<0.05),improved the pathological changes of testis,inhibited cell apoptosis,and promoted PCNA protein expression in testis.Conclusion:Wubi Shanyao pills can improve the sexual function of mice with kidney-yang-deficiency induced by hydrocortisone,which may be related to regulating the hypothalamus-pituitary-adrenal axis(HPA axis),promoting the proliferation of testicular cells,and inhibiting cell apoptosis.

关 键 词：肾阳虚 性功能 无比山药丸 小鼠 氢化可的松 

分 类 号：R285.5[医药卫生—中药学]