dsDNA修饰Ag-Thi-nGO电极作为生物传感器检测吉非替尼  

dsDNA Modified Ag-Thi-nGO Electrode as Biosensor for Detection of Gefitinib

作　　者：李洁 陈慧敏 张冰聪 俞家琪 程琼[1] LI Jie;CHEN Hui-min;ZHANG Bing-cong;YU Jia-qi;CHENG Qiong(School of Biological and Chemical Engineering,Jiaxing University,Zhejiang Jiaxing 314001,China)

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《广州化工》2021年第1期27-31,共5页GuangZhou Chemical Industry

基　　金：嘉兴学院校级重点大学生创新训练计划项目(85178559)。

摘　　要：电化学Ag-Thionine-nGO电极的表面用鲑鱼精子双链DNA(dsDNA)修饰以检测吉非替尼(Gefitinib)靶向药物与DNA的相互作用。采用差分脉冲伏安法研究Gefitinib的电化学分析过程,并评估其与固定在电极表面的dsDNA的相互作用。操作参数优化后,峰电流与Gefitinib浓度在0.10×10-5~10.00×10-5 mol/L范围内线性关系良好。The surface of the electrochemical Ag-Thionine-nGO electrode was modified with salmon sperm double-stranded DNA(dsDNA)to detect the interaction of Gefitinib-targeted drug with DNA.The electrochemical analysis of Gefitinib was studied by differential pulse voltammetry and its interaction with dsDNA immobilized on the electrode surface was evaluated.After the optimization of the operating parameters,the peak current and the Gefitinib concentration had a good linear relationship in the range of 0.10×10-5~10.00×10-5 mol/L.

关 键 词：Ag-Thionine-nGO 双链DNA生物传感器 吉非替尼 修饰电极 

分 类 号：Q625[生物学—生物物理学]