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作 者:Jun FU Shaoyong CHEN Yuanping FANG
机构地区:[1]Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization/Huanggang Normal University,Huangzhou 438000,China [2]Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains,Huangzhou 438000,China
出 处:《Agricultural Biotechnology》2020年第5期23-24,28,共3页农业生物技术(英文版)
基 金:Innovation Center Team Project of Hubei Collaborative Innovation Center for the Characteristic Resources Exploration of Dabie Mountains(2015TD07)。
摘 要:[Objectives]This study was conducted to analyze the genetic toxicity of Solidago decurrens,with a view to scientifically and safely using S.decurrens.[Methods]Using commercially available S.decurrens as an experimental material,according to Vicia faba root tip micronucleus technique,V.faba root tips were treated with different doses of aqueous extract from S.decurrens for different times,and the mitotic index,frequency of micronucleus and chromosomal aberration of root tips in V.faba were detected,so as to reveal the allelopathic potential and the potential ecological risks of S.decurrens at the cellular level.[Results]The decrease in the mitotic index caused by the aqueous extract of S.decurrens was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens increased the frequency of micronucleus in root tip cells of V.faba,and the increase was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens caused abnormal phenomena such as chromosome bridges,chromosome fragmentation,and chromosome lag in V.faba root tip cells,and the effect of the aqueous extract on V.faba root tips had significant effects dependent on time and concentration.Above results indicate that the aqueous extract of S.decurrens inhibited and damaged the mitosis of V.faba root tip cells to some degrees,had certain genetic toxicity and had the ability to rapidly spread.[Conclusions]This study provides a theoretical basis for scientifically and rationally using S.decurrens and preventing the spread of S.decurrens.
关 键 词:Solidago decurrens Genetic toxicity Vicia faba root tip MICRONUCLEUS
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