Isolation,Identification and Susceptibility Analysis of Bacillus pyocyaners from a Canine Pyometra  

作　　者：Kunyuan ZHU Lan WU Yanfei LIU Jiande YANG 

机构地区：[1]College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China

出　　处：《Agricultural Biotechnology》2020年第5期94-97,共4页农业生物技术（英文版）

基　　金：Undergraduate Innovation and Enterpreneurship Training Program of Tianjin Agricultural University(201710061193);Natural Science Foundation of Tianjin City(07JCYBJC16000)。

摘　　要：In order to clarify the causes of the fester disease in companion animals,this study involved isolation,identification and drug susceptibility analysis of pathogenic bacteria from the uterus of an 11-year-old female dog who was admitted to the veterinary hospital of Tianjin Agricultural University.According to the results of colony morphology,smear staining microscopy,physiological and biochemical reaction and 16 S rRNA gene analysis,the bacteria was identified as a Bacillus pyocyaners.The susceptibility analysis showed that the isolate is highly sensitive to aztreonam,cefdinir,cefotaxime,cefepime,ceftazidime,ofloxacin,streptomycin,kanamycin,piperacillin and tobramycin.Moreover,the isolate is moderately sensitive to ceftriaxone,cefuroxime and cefoxitin,but resistant to ampicillin,cefazolin,cefazine and medemycin.The research results provide references for controlling infection caused by B.pyocyaners.

关 键 词：Bacillus pyocyaners Isolation and identification 16S rRNA gene Susceptibility analysis 

分 类 号：S858.292[农业科学—临床兽医学]