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作 者:Yangyi MAO Huidi LUO Xi LI Peng ZHAO Jun LI Sujun WU Hongyu GUO
机构地区:[1]College of Animal Science,Shanxi Agricultural University,Taiyuan 030032,China
出 处:《Agricultural Biotechnology》2020年第5期98-100,123,共4页农业生物技术(英文版)
基 金:the Science and Technology Project of Shanxi Province(20120311024-1);Special Fund for the Construction of National Wool Sheep Industrial Technology Research System(CARS-39-24);Shanxi Provincial Science and Technology Innovation Team Fund(201705D131028-20);Shanxi Financial Fund for Agriculture(NYGX2015-03)。
摘 要:The present study was conducted to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on Texel sheep.Superovulation was conducted on 4-8-week old Texel sheep,and in-vivo ovum collection,in-vitro oocyte maturation and fertilization,and fertilized-embryo culture and transplantation were adopted to perform JIVET research on Texel sheep.The results showed that 296 available oocytes were obtained at an average of 74 oocytes per sheep;205 fertilized eggs were obtained at the 2-4 cell stage at an average of 51.25 eggs per sheep and a cleavage rate of 69.26%;and 66 of the fertilized eggs were transplanted to 11 receptor sheep.Six sheep were conceived at a conception rate of 54.55%,and 11 lambs were born.These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep.
关 键 词:Texel sheep JIVET research In-vitro fertilization 2-4-cell fertilized ovum Test-tube sheep
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