可吸收多糖止血粉体外酶解性能研究  

Study on Vitro Enzymatic Hydrolysis of Absorbable Polysaccharide Hemostatic Powder

作　　者：李玲 张敏 宋翠翠 刘连涛 LI Ling;ZHANG Min;SONG Cuicui;LIU Liantao(Saikesaisi Biotechnology Co.,Ltd.,Jinan,Shandong,250000,China)

机构地区：[1]赛克赛斯生物科技股份有限公司,山东济南250000

出　　处：《工程建设（维泽科技）》2020年第12期121-126,共6页Engineering Construction

基　　金：山东省重大科技创新工程项目可吸收创面修复生物材料研发及产业化经费支持,项目编号2019JZZY011107。

摘　　要：研究来源于淀粉的一种可吸收多糖止血粉的体外酶解性能,以评价其可降解性。模拟人体温度(37°C),在a-淀粉酶和糖化酶的协同作用下对样品进行体外酶解研究,采用3,5-硝基水杨酸(DNS)法对体外降解产生的还原糖进行测试,并从a-淀粉酶浓度、双酶活度比影响等因素确定合适的酶解条件。试验结果表明:双酶解最佳的条件为a-淀粉酶的浓度为5.6mg/ml.a-淀粉酶与糖化酶的活度比为1:7,可吸收多糖止血粉在双酶体系中降解120h后的降解率为90.13%。In order to evaluate the biodegradability of an absorbable polysaccharide hemostatic powder derived from starch,the enzymatic hydrolysis in vitro was studied.Under the synergistic action of a-amylase and glucoamylase,the in vitro enzymatic hydrolysis of the sample was studied under the simulated human temperature(37 C).The reducing sugar produced in vitro was tested by 3,5-nitrosalicylic acid(DNS)method and the suitable enzymolysis conditions were determined from the factors such as the concentration of a-amylase and the effect of double enzyme activity ratio.The results showed that the optimum conditions were as follows:the concentration of a-amylase was 5.6mg/ml,the activity ratio of a-amylase to glucoamylase was 1:7,and the degradation rate of absorbable polysaccharide hemostatic powder was 90.13%after 120 h degradation in double enzyme system.

关 键 词：可吸收多糖止血粉 a-淀粉酶 糖化酶 体外酶解 

分 类 号：R605.7[医药卫生—外科学]