Study on Virus-free and Rapid Propagation Technology of Artemisia selengensis sp.in Yangxin County  

作　　者：Shunwen XU Yihe LI Anhuai MING Rongxing YAN Xianai GAO Jin ZHENG Wei KANG 

机构地区：[1]Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation,Hubei Polytechnic University,Huangshi 435003,China [2]School of Environmental Science and Engineering,Hubei Polytechnic University,Huangshi 435003,China [3]Vegetable Office of Yangxin,Huangshi 435200,China [4]Vegetable Research Institute of Huangshi,Huangshi 435003,China

出　　处：《Agricultural Biotechnology》2020年第6期70-72,78,共4页农业生物技术（英文版）

基　　金：Hubei Polytechnic University and Yangxin Vegetable Office School-Enterprise Technology Cooperation(KY2018-049)。

摘　　要：[Objectives]This study was conducted to culture virus-free tissue culture plantlets of mid-maturing green-stalk Artemisia selengensis sp.varieties in Yangxin County.[Methods]With bud tips of A.selengensis in Yangxin as explants and MS as the basal medium,screening and proportioning of plant growth regulators were performed to establish a virus-free and rapid propagation system for mid-maturing green-stalk varieties of A.selengensis in Yangxin.[Results]The optimal callus induction medium,adventitious shoot differentiation medium,adventitious shoot elongation medium and rooting medium for explants were MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+sucrose 25 g/L+agar 7 g/L,MS+6-BA 0.1 mg/L+NAA 0.1 mg/L+sucrose 25 g/L+agar 7 g/L,MS+6-BA 0.02 mg/L+NAA 0.02 mg/L+sucrose 25 g/L+agar 7 g/L and 1/2 MS+NAA 0.05 mg/L+sucrose 25 g/L+agar 7 g/L,respectively,and the seedling rate reached more than 95%.The culture conditions were as follows:temperature(25±2)℃,relative humidity 85%,illumination intensity 3000 lx,and illumination time 14 h/d.[Conclusions]This study has important practical significance for the purification and rejuvenation and large-scale industrial breeding of Yangxin A.selengensis seedlings.

关 键 词：Artemisia selengensis sp. Culture in vitro Virus free Rapid propagation 

分 类 号：S567.239[农业科学—中草药栽培]