Separation of Molds in Contaminated Fruits and Toxicity Test  

作　　者：Qing'e LIU 

机构地区：[1]Lishui University,Lishui 323000,China

出　　处：《Agricultural Biotechnology》2020年第6期108-113,共6页农业生物技术（英文版）

基　　金：Public Welfare Technology Application Project of the Science and Technology Bureau of Lishui City(2016GYX15)。

摘　　要：[Objectives]This study was conducted to investigate the toxicity of molds in contaminative fruits.[Methods]Common fruits were used as materials to isolate contamination molds and screen the most virulent strain to study its pathogenic mechanism.[Results]We isolated eight kinds of strains from red bayberries,pears,oranges and peaches,and the P2 strain from red bayberries was the most virulent strain,which was preliminarily identified as Aspergillus luchuensis.The toxin from the strain led to the bone marrow cell micronucleus phenomenon,and the greater the toxin dose,the greater the micronucleus rate.With the extension of time,the micronucleus rate increased.The SOD activity of the kidney was inhibited after the mice were exposed to the toxin,and the greater the toxin dose,the lower the SOD activity.With the extension of time,the SOD activity decreased,but increased instead after 48 h because the toxin was metabolized in the body.It was always lower than that of control group.The MDA content in kidney was also affected after the exposure,and the greater the toxin dose,the higher the MDA content.Specifically,it increased with the time prolonged and began to decrease until 48 h,but not less than the control group.It indicated mold contamination in fruits produced toxins,and the P2 strain was the most toxic in red bayberries,causing chromosome and kidney injury.[Conclusions]This study provides a theoretical basis for the isolation and identification of molds in different fruits and toxicity tests,which is helpful to enhance people’s understanding of mycotoxins and their harm.

关 键 词：MYCOTOXIN Median lethal dose MICRONUCLEUS Cell apoptosis Super oxide dixmutase 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]