感应交流电延缓nESI中模拟生物样品引发的毛细管堵塞  

Mitigating clogging of nESI with nonvolatile buffers that mimic biological samples by induced alternative voltage

作　　者：任梦婷 黄光明[1] Ren Mengting;Huang Guangming(Department of Chemistry,School of Chemistry and Materials Science,University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]中国科学技术大学化学与材料科学学院化学系,安徽合肥230026

出　　处：《中国科学技术大学学报》2020年第11期1411-1418,共8页JUSTC

基　　金：the National Natural Science Foundation of China(21775143,21475121);the Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2017FXCX003);Users with Excellence Project of Hefei Science Center CAS(2018HSC-UE001,2018HSC-UE016);the Fundamental Research Funds for the Central Universities.

摘　　要：生物样品的检测需要纳升电喷雾离子化质谱(nESI-MS)来适应更苛刻的条件,如高浓度的非挥发性缓冲液和亚微米尺寸的毛细管孔径.以上两个需求会给nESI-MS带来一个棘手的问题:毛细管堵塞.因此,为了延缓毛细管堵塞,我们将感应交流电压施加在尖端内径小于1μm且注入高浓度盐溶液的毛细管上进行电喷雾.结果表明,改进后的毛细管比常规nESI下的毛细管寿命长1~2个数量级.较长的喷雾时间是由于电场方向周期性变化使析出的盐结晶再溶解从而延缓毛细管堵塞.同时,经过长时间(约10 min)高浓度盐溶液的电喷雾,改进后的nESI仍保持稳定和灵敏的信号.最后对模拟细胞外液和细胞内液进行了验证,结果表明感应nESI是一种延缓毛细管堵塞的广泛适用的生物样品分析方法.The detection of biological samples requires the adaptation of nanoelectrospray ionization mass spectrometry(nESI-MS)to hasher conditions,such as high concentrations nonvolatile buffers and sub-micrometer scale capillary tips.The two above-mentioned requirements would pose a considerable challenge,clogging,to nESI-MS.Herein,to mitigate the clogging problem,an induced alternative voltage was applied on the nanoemitters,which had inner diameter of less than 1μm,with the infusion of a high-concentration salt solution,to induce electrospray ionization.The tips lifetime of the modified nESI was found to be 1~2 orders of magnitude longer than that of conventional nESI.The much longer spray time could be attributed to the re-dissolution effect of salt crystals owing to the periodic change in the electric field direction.Meanwhile,the signal of the modified nESI remained stable and sensitive even after a long run(~10 min)for analysis of high-concentration salt solutions.Finally,the mimic extracellular fluid and intracellular fluid were both evaluated,and the result indicated that the application of an induced alternating current voltage can be a widely applicable method to delay clogging during biological sample analysis.

关 键 词：感应nESI 生物样品 堵塞 亚微米级毛细管 喷雾时间 

分 类 号：O657.63[理学—分析化学]