RP-HPLC法测定肾炎清片中蒙花苷的含量  

Determination of linarin in shenyanqing tablets by RP-HPLC

作　　者：刘海英 秦红霖 LIU Hai-ying;QIN Hong-lin(Pharmaceutical Instrument Supervision and Inspection Station of Zhengzhou United Logistics Support Center of PLA,Beijing 100071,China)

机构地区：[1]郑州联勤保障中心药品仪器监督检验站,北京100071

出　　处：《实用医药杂志》2021年第1期63-65,共3页Practical Journal of Medicine & Pharmacy

基　　金：军队医疗机构制剂标准提高科研专项课题(13ZJZ13-1)。

摘　　要：目的建立肾炎清片中蒙花苷的RP-HPLC含量测定方法。方法色谱柱:Agilent Zorbax SB-C18(250×4.6 mm,5μm),柱温为30℃;流动相:甲醇-0.5%醋酸溶液(50∶50),流速:1.0 ml/min;检测波长:326 nm。结果蒙花苷在0.0211~0.1053 mg/ml范围内线性关系良好(r=0.9996),平均回收率为97.1%,RSD为1.3%(n=6)。结论该方法简便、测定结果准确,重复性好,可用于肾炎清片中蒙花苷的含量测定。Objective To establish an HPLC quantitative method for the determination of linarin in shenyanqin Tablets. Methods HPLC method was used for determining the content of linarin. The chromatography column was Agilent Zorbax SB-C18(250×4.6 mm,5μm),the column temperature was 30℃. The mobile phase was methanol-0.5%acetum(50 ∶50). The flow rate was 1 ml/min and detection wavelength was 326 nm. Results The calibration curves of paeonol showed a good linear relation over the range of 0.0211-0.1053 mg/ml(r=0.9996). The average recovery of linarin was 97.1% and RSD was 1.3%(n =6). Conclusion This method is simple、accurate with good reproducibility.It can be used for the determination of linarin in Shenyanqin Tablets.

关 键 词：肾炎清片 蒙花苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]