高通量离心式液滴生成芯片设计  

Design of high throughput droplet generation chip

作　　者：周武平[1,2] 唐玉国 黎海文[1] 蒋克明[1] 刘聪[1] 张涛[1] 张志强[1] ZHOU Wu-ping;TANG Yu-guo;LI Hai-wen;JIANG Ke-ming;LI Cong;ZHANG Tao;ZHANG Zhi-qiang(Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China;University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]中国科学院苏州生物医学工程技术研究所,江苏苏州215163 [2]中国科技大学,安徽合肥230026

出　　处：《光学精密工程》2020年第12期2636-2645,共10页Optics and Precision Engineering

基　　金：吉林市与中国科学院科技合作高技术产业化专项资金资助项目(No.2019SYHZ0028);吉林省科技发展计划资助项目(No.20180201037GX);国家重点研发计划资助项目(No.2017YFF0108604)。

摘　　要：高通量单分散纳升/皮升液滴的制备在数字PCR、质谱分析、细胞筛选等生物医学领域至关重要,而现有的技术制备速率只能达到1000/s。本文提出了一种离心力与微喷嘴阵列相结合的微流控芯片,实现了超高通量液滴的制备。首先,对离心液滴微流控芯片进行设计,通过有限元仿真详细分析了微喷嘴尺寸、离心加速度和流量等参数对液滴尺寸的影响,获得了纳升级液滴芯片的设计参数,使用微纳加工方法制作离心式液滴生成芯片,并进行了液滴的高通量生成实验。实验与仿真结果表明:液滴尺寸与离心加速度成反向关系,与微喷嘴尺寸成正向关系,并在很大的流量范围内液滴尺寸基本不变,表现出优异的鲁棒性。本液滴生成芯片在离心加速度250g^500g下,可制备液滴直径为105~145μm,制备速率达27000个/s,且直径一致性良好(CV<3%),证明本液滴生成芯片具有超高通量的特点。High throughput monodisperse nano-liter/pico-liter droplet emulsions are essential in several biomedical applications,such as droplet digital PCR,mass spectrometry,and cell sorting.However,the preparation rate of existing technologies can only reach 1000/s,which restricts development of the biomedical field.We present a novel technology for providing monodisperse droplets with high throughput using centrifugal force and a micro-nozzle array.First,we designed the centrifugal droplet microfluidic chip and studied the relationships of droplet diameters with dimensions of the micro-nozzle,centrifugal acceleration,and flow rate based on simulation of the droplets’generation using the micro-nozzle;based on these,we obtained optimized design parameters for generating 1-nL droplets.Then,a microfluidic chip was fabricated using standard lithography.Finally,high throughput droplet generation was performed.The experimental and simulation results indicate that the droplet size is inversely related to the centrifugal acceleration and directly proportional to the micro-nozzle size.The droplet size is stable in a large range of flow rate,which indicates excellent robustness.Homogenous droplets with pre-selectable diameters in a range from 105 to 145μm were generated with a coefficient of variation of 3%and generation rate of 27000/s under a centrifugal acceleration range of 250g to 500g,demonstrating ultra-high throughput characteristics of the chip.

关 键 词：微流控芯片 液滴 离心 微喷嘴 高通量 

分 类 号：O652.7[理学—分析化学] O359[理学—化学]