草地贪夜蛾幼虫僵虫发生率调查及致病菌分离鉴定  

Survey on the cadaver rates of Spodoptera frugiperda and isolation of pathogenic strain

作　　者：程东美 洪婉雯 孙辉 华宏伟 张子枫 徐汉虹[2] 张志祥 CHENG Dong-Mei;HONG Wan-Wen;SUN Hui;HUA Hong-Wei;ZHANG Zi-Feng;XU Han-Hong;ZHANG Zhi-Xiang(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;College of Plant Protection,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]仲恺农业工程学院农业与生物学院,广州510225 [2]华南农业大学植物保护学院,广州510642

出　　处：《环境昆虫学报》2020年第6期1298-1304,共7页Journal of Environmental Entomology

基　　金：广东省重点领域研发计划项目(2020B020224002);广东省现代农业产业共性关键技术研发创新团队项目(2019KJ133)。

摘　　要：草地贪夜蛾Spodoptera furgiperda(J.E.Smith)是一种新的入侵害虫,目前已侵入我国多个省区,严重危害玉米。本研究调查了广州地区草地贪夜蛾僵虫的自然发生率,并分离出具有较高致病性的白僵菌,测定了其对草地贪夜蛾3龄幼虫的致病性。结果表明:广州地区草地贪夜蛾幼虫僵虫发生普遍,田间僵虫率最高为20.99%,累积僵虫率达43.21%;结合僵虫、致病菌菌落、显微超微等形态和分子生物学分析,以及致病性测定,确定从草地贪夜蛾幼虫上分离的真菌为球孢白僵菌Beauveria bassiana;分离培养的分生孢子和僵虫体表洗脱的分子孢子对幼虫致病力相近,有效致死浓度为1×10~7 cfu/mL,幼虫累积校正死亡率达78.57%。研究结果表明,该白僵菌菌株对草地贪夜蛾幼虫具有较高的致病性,可作为生防真菌进一步的研究。The fall armyworm,Spodoptera furgiperda(J.E.Smith),is a new important invasive pest.Currently,S.furgiperda has invaded many provinces in China and became an important pest of maize production.We investigated the cadaver rates of S.furgiperda in Guangzhou,and isolated the pathogenic fungi on cadaver of S.furgiperda,and tested its pathogenicity to the 3rd larvae of S.furgiperda.The results showed that the cadaver of S.furgiperda distributed widely in the cornfield;the highest natural cadaver rate could be 20.99%and cumulative cadaver rate was 43.21%.The strain was identified as Beauveria bassiana by using morphological methods,molecular biological analysis,and pathogenicity on S.furgiperda.The cultured conidium had the same mortality rates with that of cadaver surface,and the effective spore concentration was 1×107 cfu/mL,and the cumulative mortality was 78.57%at the concentration.The results indicated that strain was highly virulent to S.furgiperda and could be used as a biocontrol fungus for further research.

关 键 词：草地贪夜蛾 病原真菌 白僵菌 致病性 

分 类 号：Q968.1[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]