不同土壤类型中大豆根瘤菌解磷能力及其稳定性评价  

Evaluation of Phosphate-solubilizing Ability and Stability of Rhizobium Fredii in Various Soils

作　　者：王金生[1] 吴俊江[1] 蒲国锋[1] 刘庆莉 WANG Jin-sheng;WU Jun-jiang;PU Guo-feng;LIU Qing-li(Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences/Key Laboratory of Soybean Cultivation,Ministry of Agriculture and Rural Affairs/Heilongjiang Provincial Key Laboratory of Soybean Cultivation,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院大豆研究所/农业农村部大豆栽培重点实验室/黑龙江省大豆栽培重点实验室,黑龙江哈尔滨150086

出　　处：《大豆科学》2020年第6期906-911,共6页Soybean Science

基　　金：黑龙江省博士后科研启动金资助(LBH-Q18135);黑龙江省农科院院级科研项目专项资金(2019YYYF036);农业科技创新跨越工程专项资金(HNK2019CX01-14-6)。

摘　　要：为准确评价大豆根瘤菌解磷能力在不同土壤环境中的稳定性和适应性,筛选在黑龙江省多种土壤类型中具有高效解磷能力的大豆根瘤菌,分析前期分离、鉴定获得的10株大豆根瘤菌菌株在不同土壤类型条件下的磷解能力,并采用GGE双标图法分析评价解磷能力的稳定性。结果表明:以黑土作为“磷溶出库”,接种菌株113-2、112-1、114-2、111-2、114-1和115-2的培养基上清液无机磷含量显著高于无接种对照处理,提高幅度为0.68%~7.02%;以草甸土作为“磷溶出库”,接种菌株112-1、113-2、114-2、115-2、114-1、111-2、113-1和111-1的培养基上清液无机磷含量显著高于对照处理,提高幅度为0.57%~8.25%;以黑钙土作为“磷溶出库”,接种不同根瘤菌处理的培养基上清液无机磷含量均显著高于对照处理,提高幅度为0.75%~7.0%;以白浆土作为“磷溶出库”,接种菌株112-1、113-2、114-2、111-2、115-1、114-2、111-1和112-2的培养基上清液无机磷含量显著高于对照处理,提高幅度为0.41%~14.8%;以盐碱土作为“磷溶出库”,接种菌株113-2、112-1、114-2、111-2、114-1、115-2和113-1的培养基上清液无机磷含量显著高于对照处理,提高幅度为0.47%~9.9%。以黑土、草甸土、黑钙土、白浆土和盐碱土作为“磷溶出库”接种处理的土壤有效磷含量较对照处理分别提高0.07%~21.53%、0.08%~38.82%、0.07%~25.94%、0.27%~17.40%和0.34%~34.71%。GGE双标图数学模型综合对比得出土壤磷活化能力强且稳定性较好的菌株为112-1、113-2和114-2。In order to accurately evaluate the stability and adaptability of rhizobium fredii phosphate-solubilizing ability in different soil environment of Heilongjiang Province,this study analyzed the phosphate-solubilizing ability of 10 strains of rhizobia isolated from soybean in the pnrophase under different soil types,and evaluated the stability of phosphate solubilization ability with GGE biplot method.The results showed that the content of inorganic phosphorus in the supernatants of black soil inoculated strains 113-2,112-1,114-2,111-2,114-1 and 115-2 were significantly higher than that of uninoculated control by 0.68%-7.02%.The contents of inorganic phosphorus in the supernatants of meadow soil inoculated strains 112-1,113-2,114-2,115-2,114-1,111-2,113-1 and 111-1 were significantly higher than those of the control by 0.57%-8.25%.The content of inorganic phosphorus in the supernatants of chernozem soil inoculated different rhizobium strains were all significantly higher than that of the control by 0.75%-7.0%.The contents of inorganic phosphorus in the supernatants of albic soil inoculated strains 112-1,113-2,114-2,111-2,115-1,114-2,111-1 and 112-2 were significantly higher than those of the control by 0.41%-14.8%.The contents of inorganic phosphorus in the supernatants of saline-alkali soil inoculated strains 113-2,112-1,114-2,111-2,114-1,115-2 and 113-1 were significantly higher than those of the control,with an increase of 0.47%-9.9%.The contents of inorganic phosphorus in the supernatant of saline soil inoculated with strains 112-1,113-2,114-2,111-2,115-1,114-2,111-1 and 112-2 were significantly higher than that of control by 0.41%-14.8%.The available phosphorus content of soils inoculated with black soil,meadow soil,chernozem soil,albic soil and saline-alkali soil as phosphorus leaching reservoir increased by 0.07%-21.53%,0.08%-38.82%,0.07%-25.94%,0.27%-17.40%and 0.34%-34.71%,respectively.GGE two-plot mathematical model method was used to synthesize and compare the strains 112-1,113-2 and 114-2 which had stron

关 键 词：大豆根瘤菌 黑龙江省 解磷能力 GGE双标图 稳定性 

分 类 号：S565.1[农业科学—作物学]