维生素K3对光老化HaCaT细胞保护作用的研究  

Protective Effect of Vitamin K3 on Photoaged HaCaT Cells

作　　者：韩伊杨 魏志平 杜佳溪 陈娇 HAN Yi-yang;WEI Zhi-ping;DU Jia-xi;CHEN Jiao(Graduate School of Xuzhou Medical University,Xuzhou 221002,Jiangsu,China;Department of Dermatology,Affiliated Hospital of Xuzhou Medical University,Xuzhou 221002,Jiangsu,China)

机构地区：[1]徐州医科大学研究生院,江苏徐州221002 [2]徐州医科大学附属医院皮肤科,江苏徐州221002

出　　处：《中国美容医学》2020年第12期102-105,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨维生素K3对UVA辐照下角质形成细胞的光保护作用及相关机制。方法:以5J/cm2的UVA辐照HaCaT细胞建立光老化细胞模型。采用不同浓度(0、0.125μM、0.25μM、0.5μM)的维生素K3预处理HaCaT细胞24h;CCK-8方法检测细胞增殖活性;活性氧(ROS)检测荧光探针2',7'-二氯荧光黄双乙酸盐(DCFH-DA)检测细胞内活性氧水平;Western blot法检测各组细胞MMP1、COX2、LC3B蛋白的表达。结果:与单纯光照组相比,维生素K3能够促进光老化HaCaT细胞的体外增殖,且呈浓度依赖性,差异有统计学意义(P<0.05);药物干预组HaCaT细胞的ROS含量降低,差异有统计学意义(P<0.05);细胞内MMP1、COX2蛋白表达降低,LC3B表达增高,差异有统计学意义(P<0.05)。结论:维生素K3能够对UVA辐照HaCaT细胞提供光保护作用,其机制可能与促进细胞自噬有关。Objective To explore the photoprotective effect of Vit K3 on keratinocytes under UVA irradiation and related mechanisms.Methods HaCaT cells were irradiated with 5J/cm2 of UVA to establish a photoaging cell model.HaCaT cells were pretreated with Vit K3 at different concentrations(0,0.125μM,0.25μM,0.5μM)for 24h.Proliferation of cells was detected by CCK-8 method.Reactive oxygen species(ROS)detection fluorescent probe 2',7'-dichlorofluorescein yellow diacetate(DCFH-DA)was used to detect intracellular reactive oxygen level.The expression of MMP1,COX2 and LC3B was detected by Western blot.Results Compared with the light-only group,Vit K3 could promote the proliferation of HaCaT cells in a concentration dependent manner,the difference was statistically significant(P<0.05).The ROS content of HaCaT cells in the drug intervention group was lower(P<0.05).The expression of MMP1 and COX2 protein was decreased,and the expression of LC3B was increased(P<0.05).Conclusion Vit K3 could provide photoprotection to HaCaT cells irradiated by UVA,and its mechanism might be related to promoting autophagy.

关 键 词：HACAT细胞 光老化 紫外线A 维生素K3 自噬 

分 类 号：R339.38[医药卫生—人体生理学]