黑龙江与吉林的部分地区蜱传黄病毒的检测与鉴定  

Detection and genetic characterization of tick-borne flavivirus in parts of Heilongjiang and Jilin provinces

作　　者：蔡祥龙 闫晓敏 董馨 何彪 朱妍[1] CAI Xiang-long;YAN Xiao-min;DONG Xin;HE Biao;ZHU Yan(College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;Key Laboratory of Jilin Province for Zoonosis Prevention and Control,Military Veterinary Institute,Changchun 130122,China)

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]军事科学院军事医学研究院/军事兽医研究所/吉林省人兽共患病防控重点实验室,吉林长春130122

出　　处：《中国预防兽医学报》2020年第11期1116-1122,共7页Chinese Journal of Preventive Veterinary Medicine

基　　金：东北农业“东农学者计划”(学术骨干)项目:黑龙江省重要媒介携带病毒的病原生态学研究(19XG14)。

摘　　要：为检测黑龙江、吉林部分地区蜱传黄病毒的多样性,本研究采用布旗法、动物体表摘取法于2018年~2019年对黑龙江、吉林9个采集点进行蜱采集,应用形态学、16S rRNA鉴定蜱种类,并建立RT-PCR方法对蜱携带的黄病毒进行检测,对获得的病毒基因序列进行遗传进化分析。结果显示:采集了2402只蜱,分属于硬蜱科革蜱属、血蜱属和硬蜱属,其中森林革蜱占60.3%(1449/2402)、日本血蜱占14.1%(339/2402)、嗜群血蜱占8.0%(192/2402)和全沟硬蜱占17.6%(422/2402)。从黑龙江全沟硬蜱中检测到阿龙山病毒(ALSV),扩增到1531 nt的NS3-like基因;吉林日本血蜱中检测到恩格耶病毒(NGOV),扩增到916 nt的NS5基因;吉林省森林革蜱中检测到蜱传脑炎病毒(TBEV),获得11051 nt该全长病毒基因组。遗传进化分析显示,ALSV与黑龙江ALSV H3株属于同一进化分支,NGOV是一种新发现的蜱传黄病毒,所获得的TBEV属远东型,与疫苗株同源性为93.86%。本研究初步揭示了该地区的优势蜱种分布,并首次发现了蜱传新型黄病毒NGOV,为该地区蜱传黄病毒的流行情况提供了重要的基础数据。In order to reveal the diversity of tick-borne flavivirus(TBFV)in Heilongjiang and Jilin provinces.In this study,ticks were collected using drag-flag or picking directly from infested animals from nine locations during 2018-2019,and subjected to species identification based on their morphology and 16S rRNA.TBFVs were detected using RT-PCR method and genetically analyzed to infer their phylogeny.Our results showed that a total of 2402 ticks were collected,covering Dermacentor silvarum 60.3%(1449/2402),Haemaphysalis japonica 14.1%(339/2402),Haemaphysalis concinna 8.0%(192/2402)and Ixodes persulcatus 17.6%(422/2402).Among these TBFVs,Alongshan virus was detected from I.persulcatus in Heilongjiang province,and a 1531 ntlong NS3-like gene was amplified.Ngoye virus was detected from H.japonica in Jilin province,and a 916 nt-long fragment of NS5 gene was amplified.Tick-borne encephalitis virus was detected from D.silvarum in Jilin province,and its complete genome(11051 nt)was successfully obtained.Phylogenetic analyses showed that Alongshan virus closely clustered with Heilongjiang strain H3 and Ngoye virus was a newly discovered tick-borne flavivirus.Meanwhile,tick-borne encephalitis virus belonged to the Far Eastern type and had a homology of 93.86%with the vaccine strain.This study preliminarily revealed the distribution of dominant tick species in this area and discovered the tick-borne novel flaviviruses Ngoye virus for the first time,thus enhanced our understanding of the diversity of tick-borne flavivirus in the region.

关 键 词：蜱 黄病毒 遗传多样性 森林脑炎病毒 

分 类 号：S852.65[农业科学—基础兽医学]