基于非洲菊转录组测序的SSR位点信息分析  

Analysis on SSR Loci Information in the Transcriptome Sequencing of Gerbera Jamesonii L.

作　　者：林发壮 李锦烨 安慧珍 黄琦 姚凤琴 钟琳珊 陈昌铭 郭芸玮 LIN Fa-zhuang;LI Jin-ye;AN Hui-zhen;HUANG Qi;YAO Feng-qin;ZHONG Lin-shan;CHEN Chang-ming;GUO Yun-wei(Institute of Sanming Agricultural Sciences, Sanming, Fujian 365509, China;Sanming Forest Seedling Management Station, Sanming, Fujian 365000, China;Xiamen Agricultural Technology Extension Center, Xiamen, Fujian 361009, China)

机构地区：[1]三明市农业科学研究院,福建三明365509 [2]三明市林木种苗站,福建三明365000 [3]厦门市农业技术推广中心,福建厦门361009

出　　处：《福建农业科技》2020年第11期1-6,共6页Fujian Agricultural Science and Technology

基　　金：三明市林业科学研究项目(明林计财〔2017〕49号);福建省科技计划星火项目(2018S0031);2019年度中央引导地方科技发展专项资金项目(2019L3025)。

摘　　要：为深入开发非洲菊SSR分子标记,进而推动非洲菊遗传多样性研究,以云南红非洲菊的转录组序列为基础,对SSR位点进行挖掘。结果表明:通过MISA软件从60563条unigenes检测出14928个SSR位点,分布于11932条unigenes中,出现频率为24.65%,平均分布距离为3.43 kb。优势重复基序为单核苷酸、二核苷酸和三核苷酸,分别占总SSR位点的63.44%、21.54%和14.06%。14928个SSR位点由73种重复基序构成,(A/T)、(AG/CT、AC/GT)、(AAT/ATT、AAG/CTT)分别是单核苷酸、二核苷酸和三核苷酸优势重复基元,分别占总SSR重复类型的62.31%、18.13%和6.93%。利用Primer 3.0对SSR序列设计引物9535对,成功率为63.87%。非洲菊转录组SSR位点丰富,分布密度大,具有较高的多态性潜能,可作为开发SSR标记的有效来源,为非洲菊遗传多样性研究,分子标记辅助育种、遗传图谱构建以及功能基因挖掘等提供科学依据。In order to further develop the SSR molecular markers and promote the researches on the genetic diversity of Gerbera jamesonii L.,the SSR loci were mined on the basis of the transcriptome sequencing of Yunnan Red Gerbera jamesonii L..The results showed that 14928 SSR sites were detected from 60563 unigenes by the MISA software,which were distributed among 11932 unigenes,with a frequency of 24.65%and an average distribution distance of 3.43 kb.The predominant repeat motifs were mononucleotide,dinucleotide,and trinucleotide,which accounted for 63.44%,21.54%,and 14.06%of the total SSR loci,respectively.A total of 14928 SSR loci consisted of 73 kinds of repeat motifs.Among them,A/T,AG/CT,AC/GT,AAT/ATT,and AAG/CTT were the predominant repeat motifs of mononucleotide,dinucleotide,and trinucleotide,accounting for 62.31%,18.13%,and 6.93%of the total SSR repeats,respectively.Primer 3.0 was used to design 9535 pairs of SSR sequencing primers,and the success rate was 63.87%.Therefore,Gerbera jamesonii L..had abundant SSR loci with transcription sites,large distribution density,and excellent polymorphism potential.It could be used as an effective source for the development of SSR markers,and would provide scientific basis for the study of genetic diversity,molecular marker-assisted breeding,genetic map construction,and functional gene mining,etc.

关 键 词：非洲菊 转录组 SSR引物 位点信息 

分 类 号：S682.1[农业科学—观赏园艺]