基于尿囊素及饮片性状变化的麸炒山药炮制工艺量化优选  

Quantitative optimization of processing technology of Dioscoreae rhizoma stir-baked with wheat bran based on allantoin and changes of traits of decocting pieces

作　　者：郑美娟 叼全红 ZHENG Meijuan;DIAO Quanhong(Department of Pharmacy,Zhangzhou Health Vocational College,Zhangzhou,Fujian,363000,China;Inspection Section,Zhangzhou Market Supervision Bureau,Zhangzhou,Fujian,363000,China)

机构地区：[1]漳州卫生职业学院药学系,福建漳州363000 [2]漳州市市场监督局稽查科,福建漳州363000

出　　处：《甘肃中医药大学学报》2020年第6期46-50,共5页Journal of Gansu University of Chinese Medicine

基　　金：福建省高等学校新世纪人才支持计划(闽教科〔2017〕52号);漳州市科技局科技重大专项(ZZ2018ZD2019)。

摘　　要：目的优选麸炒山药的最佳炮制工艺。方法以麸炒后山药饮片的外观性状和尿囊素含量作为考察指标,以预热锅温、炒制时间、麦麸用量及翻炒频率作为考察因素,采用正交试验与多指标综合评分法,优选麸炒山药的最佳炮制工艺;采用高效液相色谱(HPLC)法测定麸炒山药中尿囊素的含量,色谱柱为C_(18)(250.0 mm×4.6 mm,5μm),流动相为甲醇∶水(1∶99),流速1 m L/min,柱温30℃,检测波长220 nm,进样量20μL。结果尿囊素的回归方程为Y=2830.5X+1107.5,r=0.9996,线性范围为10~50μg/m L;优选的最佳工艺为A2B1C2D3,即预热锅温160℃,炒制时间3 min,麦麸用量15%,翻炒频率每分钟80次;验证了3批麸炒山药样品,结果性状评分分别为5.0,5.0,5.5分,尿囊素含量分别为0.30%,0.31%,0.29%。结论优选的炮制工艺稳定、合理、可行,可为麸炒山药饮片的产业化提供参考。Objective To optimize the processing technology of Dioscoreae rhizoma stir-baked with wheat bran.Methods By appearance traits of decocting pieces of Dioscoreae rhizoma stir-baked with wheat bran and allantoin content as the index of evaluation,by the preheating pot temperature,the stir-baking time,the amount of wheat bran and the frequency of stir-baking as the factors of evaluation,the orthogonal test and multi-index comprehensive scoring method were used to optimize the best processing technology of Dioscoreae rhizoma stir-baked with wheat bran.High performance liquid chromatography(HPLC)method was used to determine allantoin content in Dioscoreae rhizoma stir-baked with wheat bran on C18(250.0 mm×4.6 mm,5μm),the mobile phase was a mixture of methanol and water(1∶99),with flow rate 1 m L/min and column temperature 30℃,the detection wavelength was 220 nm,and the sample amount injected was 20μL.Results The regression equation of allantoin was Y=2830.5 X+1107.5,r=0.9996,and the linear range was 10 to 50μg/m L.The optimal process was A2B1C2D3,that is,preheating pot temperature 160℃,stir-baking time 3 min,the amount of wheat bran 15%,stir-baking frequency 80 times per minute.Three batches of Dioscoreae rhizoma stir-baked with wheat bran samples were verified,the results showed that the trait scores were 5.0,5.0,5.5,and the allantoin content were 0.30%,0.31%and 0.29%,respectively.Conclusion The optimized processing technology is stable,reasonable and feasible,which provides references for the industrialization of decocting pieces of Dioscoreae rhizoma stir-baked with wheat bran.

关 键 词：山药 尿囊素 麸炒 正交工艺 高效液相色谱 含量测定 

分 类 号：R283[医药卫生—中药学]