基于非靶向气相色谱-质谱技术探讨杜仲-刺蒺藜药对降低老年自发性高血压大鼠血压的粪便代谢组学研究  

Fecal Metabonomics Study of Duzhong(Eucommia ulmoides Oliv.)-Ezhu(Tribulus terrestris L.) on Reducing Blood Pressure in Elderly Spontaneously Hypertensive Rats Based on Non-Targeted Gas Chromatography-Mass Spectrometry

作　　者：亓英姿 姜月华[2] 杨传华[3] QI Yingzi;JIANG Yuehua;YANG Chuanhua(Health College of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China;Central Laboratory of Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China;Department of Cardiology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China)

机构地区：[1]山东中医药大学健康学院,山东济南250014 [2]山东中医药大学附属医院中心实验室,山东济南250014 [3]山东中医药大学附属医院心病科,山东济南250014

出　　处：《中华中医药学刊》2020年第12期131-134,I0042,I0043,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81573916,81673807);泰山学者岗位建设基金(2018-35)。

摘　　要：目的观察杜仲-刺蒺藜对自发性高血压大鼠(SHRs)的降压效应及其对代谢组学的影响。方法选用32只18月龄雄性SHRs,将其随机分为对照组和杜仲-刺蒺藜药对干预高、中、低剂量组共4组,灌胃给药8周。每周测血压,观察结束后遵循无菌操作原则取大鼠应激粪便样本,对粪便样本进行非靶向气相色谱-质谱法代谢物检测。结果与对照组比较,杜仲-刺蒺藜药对中剂量组对清醒SHRs具有良好的降压作用(P<0.05),上调了乙醇酸、鸟嘌呤、二乙酸、色胺代谢水平。结论杜仲-刺蒺藜药对具有确切的降压作用,可改变老龄SHRs内源性代谢物。Objective To observe the effects of Duzhong(Eucommia ulmoides Oliv.)-Tribulus terrestris L.(ET) on the fecal metabonomics by non-targeted Gas Chromatography-Mass Spectrometry(GC-MS) in spontaneously hypertensive rats(SHRs) to explore the anti-hypertensive mechanism. Methods Thirty-two 18-month-old male SHRs were randomly divided into 4 groups: SHR group and high, medium and low dose of ET groups. Rats were intragastrically administrated for 8 weeks. Blood pressure was measured every week. The stress stool samples were taken out according to the aseptic operation principle. The non-targeted GC-MS was used to analyzed fecal metabolites. Results Compared with the untreated SHRs, ET had a good anti-hypertensive effect(P<0.05), and increased the metabolic levels of glycolic acid, guanine, diacetic acid and tryptamine. Conclusion ET has an effective anti-hypertensive effect and can change the endogenous metabolites of aged SHRs.

关 键 词：杜仲-刺蒺藜 非靶向气相色谱-质谱代谢组 自发性高血压大鼠 

分 类 号：R285.5[医药卫生—中药学]