番泻叶和大青叶药材的荧光鉴别  

Fluorescence Identification of Fanxieye(Sennae Folium)and Daqingye(Isatidis Folium)

作　　者：王鑫瑞 冯帅[1] 李峰[1] WANG Xinrui;FENG Shuai;LI Feng(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《山东中医药大学学报》2021年第1期125-132,共8页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省重点研发计划项目(编号:2016GSF202012);山东省中医药科技发展计划项目(编号:2017-024);山东中医药大学SRT项目(编号:2017043)。

摘　　要：目的:用荧光法鉴别叶类中药材大青叶和番泻叶。方法:通过在紫外光灯下观察原药材和药材浸出液的荧光,以及使用荧光分光光度计扫描药材浸提液的荧光光谱,鉴别大青叶和番泻叶。结果:紫外光灯下(365 nm),相同溶剂的番泻叶和大青叶浸出液的荧光有明显区别;在激发波长λ=365 nm时,两种药材的荧光峰强度有显著差异;在激发波长λ=254 nm时,两种药材的峰形和荧光峰强度均有显著差异。结论:荧光法鉴别番泻叶和大青叶方法简便,且较为可靠。Objective:To identify Fanxieye(Sennae Folium)and Daqingye(Isatidis Folium)with leaves used as medicinals by fluorescence method.Methods:Fanxieye and Daqingye were identified by observing the fluorescence of crude medicinal herbs and extracts under ultraviolet lamp,and scanning the fluorescence spectrum of extracts with fluorescence spectrophotometer.Results:Under ultraviolet light(365 nm),the fluorescence of Fanxieye and Daqingye with the same solvent was significantly different.The fluorescence peak intensity of the two medicinal materials was significantly different at excitation wavelengthλ=365 nm.The peak shape and fluorescence peak intensity of the two medicinal materials differ significantly at excitation wavelength ofλ=254 nm.Conclusion:Fluorescence is a simple and reliable method for the identification of Fanxieye and Daqingye.

关 键 词：荧光光谱 荧光分光光度计 番泻叶 大青叶 激发波长 荧光峰强度 中药研究 

分 类 号：R282.5[医药卫生—中药学]