Integrated transcriptomic and proteomic study on the different molecular mechanisms of PC12 cell growth on chitosan and collagen/chitosan films  

作　　者：Xiaoying Lu Yan Huang Yayun Qu Yiwen Zhang Zequn Zhang 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China [2]SQ Medical Device Co.,Ltd,Nanjing 210008,China

出　　处：《Regenerative Biomaterials》2020年第6期553-565,共13页再生生物材料（英文版）

基　　金：the support of the National Natural Science Foundation of China(31271012,31971254,31600816);the Natural Science Foundation of Jiangsu Province(BK20150599).

摘　　要：The purpose of this article is to integrate the transcriptomic analysis and the proteomic profiles and to reveal and compare the different molecular mechanisms of PC12 cell growth on the surface of chitosan films and collagen/chitosan films.First,the chitosan films and the collagen/chitosan films were prepared.Subsequently,the cell viability assay was performed;the cell viability of the PC12 cells cultured on the collagen/chitosan films for 24 h was significantly higher than that on the chitosan films.Then,with cDNA microarray,the numbers of differentially expressed genes of PC12 cells on the surface of chitosan and collagen/chitosan films were 13349 and 5165,respectively.Next,the biological pathway analysis indicated that the differentially expressed genes were involved in 40 pathways directly related to cell adhesion and growth.The integrated transcriptomic and our previous proteomic analysis revealed that three biological pathways-extracellular matrix-receptor interaction,focal adhesion and regulation of actin cytoskeleton-were regulated in the processes of protein adsorption,cell adhesion and growth.The adsorbed proteins on the material surfaces further influenced the expression of important downstream genes by regulating the expression of related receptor genes in these three pathways.In comparison,chitosan films had a strong inhibitory effect on PC12 cell adhesion and growth,resulting in the significantly lower cell viability on its surface;on the contrary,collagen/chitosan films were more conducive to promoting PC12 cell adhesion and growth,resulting in higher cell viability.

关 键 词：chitosan film and collagen/chitosan film protein adsorption and cell growth cDNA microarray and proteomics biological pathways 

分 类 号：Q51[生物学—生物化学]