VEGF在门静脉高压患者术后门静脉系统血栓形成过程中的表达及意义  

Significance of the expression of VEGF in postoperative portal venous thrombosis in patients with portal hypertension

作　　者：刘军 胡洪生 吴红伟 付海峰 魏广民 胡伟 孙少华 LIU Jun;HU Hongsheng;WU Hongwei;FU Haifeng;WEI Guangmin;HU Wei;SUN Shaohua(Department of Hepatopancreatobiliary,Breast and Thyroid Surgery,Dongfeng Hospital Affiliated to Hubei Medicial College,Shiyan,Hubei 442008,P.R.China)

机构地区：[1]湖北医药学院附属东风医院肝胆胰乳腺甲状腺外科,湖北十堰442008

出　　处：《中国普外基础与临床杂志》2021年第1期63-66,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)在肝硬变门静脉高压患者术后门静脉系统血栓形成过程中的表达及意义。方法收集2014年1月至2018年12月期间在湖北医药学院附属东风医院就诊的146例肝硬变门静脉高压患者血清及手术切除的脾静脉及脾脏标本,用酶联免疫吸附法测定血清VEGF水平,用免疫组织化学方法检测脾静脉及脾脏组织中VEGF的表达情况,依据术后是否形成门静脉系统血栓分为血栓形成组和非血栓形成组,对比2组间的差异。结果肝硬变门静脉高压患者术后血栓形成组血清VEGF水平明显高于非血栓形成组(P<0.05);脾静脉壁及脾脏组织中,血栓形成组的VEGF染色指数明显高于非血栓形成组(P<0.05)。结论门静脉高压患者术后门静脉系统血栓可能与血清VEGF水平有关,脾静脉壁及脾脏VEGF高表达,提示VEGF可能参与了门静脉系统血栓的形成过程。Objective To investigate the significance of the expression of vascular endothelial growth factor(VEGF)in portal vein thrombosis after operation in patients with portal hypertension.Methods The serum of 146 patients with portal hypertension treated in Dongfeng Hospital Affiliated to Hubei Medicial College from January 2014 to December 2018 and the surgically removed splenic vein and spleen specimens were collected.The serum VEGF level was determined by enzyme-linked immunosorbent assay,and the expressions of VEGF in splenic vein and spleen tissues were detected by immunohistochemistry.According to whether portal vein thrombosis was formed after operation,the patients were divided into thrombosis group and non-thrombosis group,and the differences between the groups were compared.Results The serum VEGF level in the thrombosis group was significantly higher than that in the non-thrombosis group(P<0.05).In splenic vein wall and spleen tissues,VEGF staining indexes in the thrombosis group were significantly higher than those in the non-thrombosis group(P<0.05).Conclusions Postoperative portal vein thrombosis in patients with portal hypertension may be related to the serum VEGF level.The high expressions of VEGF in splenic vein wall and spleen suggest that VEGF may participate in the formation process of portal vein thrombosis.

关 键 词：门静脉高压 血栓 血管内皮生长因子 血小板 

分 类 号：R657.3[医药卫生—外科学]