新型植物免疫诱抗剂ZNC对烟草的促生抗病效果  

Application Effect of a Novel Plant Immune Inducer ZNC in Tobacco

作　　者：郭梅燕 刘保友[1,4] 李洋[1] 张晓英[2] 陈玉国 丁新华[1] GUO Mei-yan;LIU Bao-you;LI Yang;ZHANG Xiao-ying;CEHN Yu-guo;DING Xin-hua(College of Plant Protection,Shandong Agricultural University,Tai’an 271018;Shandong Pengbo Biotechnology Co.Ltd.,Tai’an 271000;Henan Xiangcheng Agricultural Science Research Institute,Xiangcheng 461700;Yantai Agricultural Sci&Tech Institute,Yantai 265500;Tobacco Research Institute,Henan Academy of Agricultural Sciences,Xuchang 461000)

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]山东蓬勃生物科技有限公司,泰安271000 [3]河南省项城市农业科学研究所,项城466200 [4]山东省烟台市农业科学研究院,烟台265500 [5]河南省农业科学院烟草研究所,许昌461000

出　　处：《生物技术通报》2021年第1期182-188,共7页Biotechnology Bulletin

基　　金：山东省重点研发计划(2019JZZY020608,2019GNC106152);国家自然科学基金项目(31872925);山东省自然科学杰出青年基金(JQ201807);山东省高等学校青创科技支持计划(2019KJF023)。

摘　　要：免疫诱抗剂智能聪(ZNC)是一种宛氏拟青霉乙醇提取物,具有促进植物生长提高植物抗逆的能力。本文在烟草不同生长期喷施ZNC,分析其诱导烟草抗病效果及对烟草生长发育的影响。结果表明,3个浓度的ZNC均对病毒病表现出良好防效,且减轻了根茎类病害和后期叶斑类病害的发生程度,其中500 ng/mL ZNC抗病毒病、赤星病等病害的效果最好,抗病毒效果可达57.07%,表明该诱抗剂对烟草主要病害都有较好的诱抗效果。同时,50 ng/mL和500 ng/mL处理分别较CK增产6.00%和5.41%,且500 ng/mL还增加了总糖、还原糖、总氮、K2O等指标的含量,表现出了一定的增产提质效果。试验还表明,尽管1000 ng/mL ZNC表现出抗病效果,但对烟草生长表现出显著的抑制作用,说明该诱抗剂在一定的剂量范围内对烟草有较好促生抗病效果,仍需要进一步试验来确定。Immune inducer ZhiNengCong(ZNC),an ethanol extract of endophyte Paecilomyces variotii,has function of promoting plant growth and enhancing disease resistance.Our study aims to evaluate the induced disease resistance and growth and development effect of ZNC on tobacco while ZNC were sprayed at the different stage of tobacco.The results showed that the 3 concentrations of ZNC showed fine control effect on viral diseases,and reduced the occurrence of rhizome diseases and later leaf spot diseases.The control effect of 500 ng/mL ZNC on tobacco virus and brown spot was the best,reaching 57.07%,indicating that this inducer had a favorable inducement effect on the main tobacco diseases.Compared with CK,the yield increased 6.00%and 5.41%respectively in 50 ng/mL and 500 ng/mL treatments,and the content of total sugar,reducing sugar,total nitrogen and K2O also increased in 500 ng/mL,i.e.,increasing yield and improving quality occurred at certain level.More experiment demonstrated that 1000 ng/mL ZNC treatment showed anti-disease effect,however,it presented significantly inhibitory effect on tobacco growth.This showed that ZNC had a fine effect on tobacco growth and disease resistance within a certain dose range,and further experiments are needed to determine it.

关 键 词：免疫诱抗剂 ZNC 烟草 抗病性 生长发育 

分 类 号：S572[农业科学—烟草工业]