叶绿素结合蛋白CP24介导光照响应基因StRSM 1调控叶绿素积累  

Light-responding Gene StRSM 1 Mediated by Chlorophyll-binding Protein CP24 Regulates Chlorophyll Accumulation

作　　者：撒世娟 伍涵宇 张晓萍[1] 郑蕊[1,2] 姚新灵[1,2] SA Shi-juan;WU Han-yu;ZHANG Xiao-ping;ZHENG Rui;YAO Xin-ling(Lab Molecular Breeding for Local Crops in Ningxia,Ningxia University,Yinchuan 750021;Education Ministry Lab for Protection and Utilization on Bioresource in the West China,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏优势特色作物现代分子育种重点实验室宁夏大学,银川750021 [2]西部特色生物资源保护及利用教育部重点实验室宁夏大学,银川750021

出　　处：《生物技术通报》2021年第1期198-204,共7页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31160236,31960123);宁夏农业育种专项(2014NYYZ02)。

摘　　要：单MYB转录因子成员RSM(RADIALIS-like SANT/MYB)突变,倒置黑暗诱导的叶绿素减少,原因有待确定;为了揭示RSM如何调控叶绿素积累,本研究运用基因工程途径,获得了普通烟草和马铃薯体内抑制和过量表达StRSM 1阳性转化株系,测定了阳性转化株系的叶绿素积累等生理表型;结果显示,普通烟草和马铃薯体内抑制RSM 1表达,显著增加了叶绿素积累,叶色随之加深;RSM 1过量表达,显著减少叶绿素积累,叶色变浅。叶绿素代谢相关基因表达测定结果显示,StRSM 1过量表达增加了黑暗下叶绿素结合蛋白CP24基因的表达,改变了其表达模式。以上结果表明,转录因子StRSM 1响应光照反向调控叶绿素积累,叶绿素结合蛋白CP24参与了StRSM 1对叶绿素积累的调控。结果有助于进一步明确RSM 1如何响应光照和深刻理解RSM 1参与的光照响应。Mutation on RSM(RADIALIS-like SANT/MYB),a member of the single MYB transcription factor family inverts darknessinduced chlorophyll reduction,and the reason for it is unknown yet.To reveal how RSM regulates chlorophyll accumulation,StRSM 1-overexpressed positive transformant line of Nicotiana tabacum and Solanum tuberosum were generated,and chlorophyll accumulation and other physiologic phenotypes were assayed.The result showed that over-expression of RSM1 led to light green leaf and a less chlorophyll accumulation,and inhibition of RSM 1 expression increased chlorophyll accumulation.Expression assay of genes encoding chlorophyll metabolism and binding demonstrated that over-expression of StRSM 1 increased the expression of chlorophyll-binding protein CP24 under darkness and changed expression pattern of CP24.The above findings indicate that light-responding transcription factor StRSM 1 negatively regulates chlorophyll accumulation.CP24 involves in the regulation.The result will play an important role in further clarifying how RSM 1responds to light and deeply understanding the light response involved in RSM1.

关 键 词：StRSM 1 叶绿素结合蛋白 叶绿素 

分 类 号：Q943.2[生物学—植物学]