基于TGF-β/smad信号通路探讨七方胃痛颗粒对人胃腺癌AGS细胞上皮间质转化的影响  

Effect of Qifang Weitong Granule on Human Gastric Adenocarcinoma AGS-EMT Cells Based on TGF-β/Smad Signaling Pathway

作　　者：袁铁超 陈国忠[2] 彭振西 易志忠 欧智海 YUAN Tiechao;CHEN Guozhong;PENG Zhenxi;YI Zhizhong;OU Zhihai(Guangxi University of Chinese Medicine,Nanning Guangxi China 530001;The First Afiliated Hospital of Guangxi University of Chinese Medicine,Nanning Guangxi China 530023)

机构地区：[1]广西中医药大学,广西南宁530001 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中医学报》2021年第1期126-131,共6页Acta Chinese Medicine

基　　金：国家自然科学基金项目(81560758)。

摘　　要：目的:基于TGF-β/smad信号通路探讨七方胃痛颗粒对人胃腺癌AGS-EMT细胞ZEB2、E-cad及Vimentin的影响及可能的机制。方法:用TGF-β1处理AGS细胞建立上皮间质转化(epithelial mesenchymal transition,EMT)模型,将细胞分为空白组、七方胃痛颗粒(含药血清+EMT模型)组、LY2109761(TGF-β/Smad抑制剂+EMT模型)组,用RT-PCR、Western Blot法检测ZEB2、E-cad及Vimentin的表达。结果:与空白组比较,七方胃痛颗粒及LY2109761干预后能上调E-cad蛋白及其mRNA的表达,下调ZEB2蛋白及其mRNA和Vimentin蛋白及其mRNA的表达(P<0.05),并呈时间依赖性。与七方胃痛颗粒组相比,LY2109761组ZEB2蛋白及其mRNA的表达明显下调(P<0.05)。结论:七方胃痛颗粒与LY2109761作用相似,其可能是通过抑制TGF-β1/Smad信号通路,上调E-cad表达,下调ZEB2及Vimentin表达,抑制EMT形成,从而抑制人胃腺癌AGS细胞的上皮-间质转化。Objective:To investigate the effects of Qifang Weitong Granule on zinc finger E-box-binding protein 2(ZEB2),E-cadherin(E-cad)and vimentin in human gastric adenocarcinoma ags-emt cells based on TGF-β/smad signaling pathway.Methods:AGS cells were treated with TGF-β1 to establish EMT model.The cells were divided into blank group,Qifang Weitong Granule(drug containing serum+EMT model)group and LY2109761(TGF-β/Smad inhibitor+EMT model)group.RT-PCR and Western Blot were used to detect the expression of ZEB2,E-cad and vimentin.Results:Compared with the blank group,Qifang Weitong Granule and LY2109761 could up regulate the expression of E-cad protein and its mRNA,down regulate the expression of ZEB2 protein and its mRNA,vimentin protein and its mRNA in a time-dependent manner(P<0.05).Compared with Qifang Weitong Granule group,the expression of ZEB2 protein and its mRNA in LY2109761 group was significantly decreased(P<0.05).Conclusion:Qifang Weitong Granule has similar effect as LY2109761.It may inhibit epithelial mesenchymal transition of human gastric adenocarcinoma AGS cells by inhibiting TGF-β1/Smad signaling pathway,up regulating the expression of E-cad,down regulating the expression of ZEB2 and vimentin,and inhibiting the formation of EMT,thereby inhibiting the epithelial-mesenchymal transition of human gastric adenocarcinoma AGS cells.

关 键 词：七方胃痛颗粒 人胃腺癌细胞 上皮间质转化 ZEB2 E-CAD VIMENTIN LY2109761 

分 类 号：R285.5[医药卫生—中药学]