Shewanella marisflavi EP1高盐度下呼吸2,4-二硝基甲苯的全基因组分析  

Complete Genome Analysis of Shewanella marisflavi EP1 Associatedwith 2,4-Dinitrotoluene Respiration under High Salinity

作　　者：黄杰勋 王汉林 黄卫东 刘先利 HUANG Jiexun;WANG Hanlin;HUANG Weidong;LIU Xianli(School of Environmental Science and Engineering,Huangshi Hubei 435003;Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation,Huangshi Hubei 435003;School of Chemistry and Chemical Engineering,Hubei Polytechnic University,Huangshi Hubei 435003)

机构地区：[1]湖北理工学院环境科学与工程学院,湖北黄石435003 [2]湖北理工学院矿区环境污染控制与修复湖北省重点实验室,湖北黄石435003 [3]湖北理工学院化学与化工学院,湖北黄石435003

出　　处：《湖北理工学院学报》2021年第1期9-14,共6页Journal of Hubei Polytechnic University

基　　金：湖北省科技厅技术创新专项重大项目(项目编号:2016ACA176);湖北理工学院引进人才项目(项目编号:201710920043)。

摘　　要：工业废水中通常含有大量的盐类,而二硝基甲苯(DNT)在好氧条件下较难降解,因此在高盐度条件下对二硝基甲苯进行厌氧生物转化是一种极具潜力的废水处理方法。在高盐度条件下通过EP1对2,4-DNT的呼吸还原能力进行测定,发现Shewanella marisflavi EP1对2,4-DNT具有高效的还原转化能力及较高的耐盐性。在NaCl浓度高达10%时,通过呼吸作用能将2,4-DNT进行还原转化。全基因组测序表明,菌株EP1包含1个约4.3 Mbps的环状染色体,其G+C含量为50%。基因组分析进一步证实,EP1含有多个参与电子转移到胞外受体和耐盐性相关的基因。Due to the presence of large amounts of salts in many industrial effluents and the recalcitrance of dinitrololuene(DNT)to aerobic degradation,anaerobic biotransformation of DNT under high salinity is a potential way to remediate the DNT contaminated wastewater.By using EP1 to measure the respiratory reduction capacity of 2,4-DNT under high salinity conditions,it was found that excellent 2,4-DNT transformation performance and saline tolerance were demonstrated with S.marisflavi EP1.The reductive transformafion can be done for 2,4-DNT by respiration when NaCl concentration reached 10%.The complete genome sequence of strain EP1 contained a circular chromosome of~4.3 Mbps with 50%G+C content.Genome analysis further confirmed that EP1 contained many genes associated with electron reached to extracellular acceptors and salinity tolerance.

关 键 词：希瓦氏菌 生物转化 耐盐性 基因组 2 4-二硝基甲苯 

分 类 号：X783[环境科学与工程—环境工程] Q789[生物学—分子生物学]