山豆根内生菌发酵苦参的化学成分研究  

Research on chemical composition of sophora flavescens fermented by endophyte from radix sophorae tonkinensis

作　　者：宋娜丽[1,2] 张苇 张毅 张菊 杨丽萍[5] 马克坚 蔡乐[2] 赵霞[1] SONG Nali;ZHANG Wei;ZHANG Yi;ZHANG Ju;YANG Liping;MA Kejian;CAI Le;ZHAO Xia(Central Laboratory,Yunnan Institute of Traditional Chinese Medicine,Kunming 650223,China;Key Laboratory of Natural Resources Medicinal Chemistry in Ministry of Education,School of Chemical Science and Technology,Yunnan University,Kunming 650091,China;College of Vocational and Technical Education,Yunnan Normal University,Kunming 650091,China;KPC Pharmaceuticals,Inc,Kunming 600422,China;Yunnan Key Laboratory of Dai and Yi Medicines,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南省中医中药研究院中心实验室,云南昆明650223 [2]云南大学化学科学与工程学院,自然资源药物化学教育部重点实验室,云南昆明650091 [3]云南师范大学职业技术教育学院,云南昆明650091 [4]昆药集团股份有限公司,云南昆明600422 [5]云南中医药大学云南省傣医药与彝医药重点实验室,云南昆明650500

出　　处：《中国现代医生》2020年第33期48-54,共7页China Modern Doctor

基　　金：云南省中医中药研究院院级项目(zyy2018015,zyy2018018);云南省科技厅面上项目(2019FB120);国家自然科学基金项目(81960760)。

摘　　要：目的研究苦参经山豆根内生菌发酵后,其化学成分的变化,分析山豆根内生真菌对苦参化学成分变化的影响。方法利用硅胶柱色谱、薄层层析硅胶板从发酵苦参中分离得到7个化合物并利用波谱技术鉴定其结构。结果从发酵的苦参中分离得到7个化合物,分别是1H-3-醛基-吲哚(1)、β-谷甾醇(2)、苦参碱(3)、槐定碱(4)、N-苯乙基乙酰胺(5)、氧化苦参碱(6)、胡萝卜苷(7)。结论1H-3-醛基-吲哚(1)、N-苯乙基乙酰胺(5)首次从发酵苦参中得到,可能为发酵后产生的化合物。Objective To research the changes of chemical composition of sophora flavescens after fermentation by endophyte from radix sophorae tonkinensis,and to analyze the impacts of endophytic fungi from sophora tonkinensis on the changes of chemical composition of sophora flavescens.Methods Seven compounds were isolated from fermented sophora flavescens by silica gel column chromatography and thin layer chromatography silica gel plate,and their structures were identified by spectrum technology.Results Seven compounds were isolated from fermented sophora flavescens,which were 1H-3-aldehyde-indole(1),β-sitosterol(2),matrine(3),sophoridine(4),N-phenylethylacetamide(5),oxymatrine(6)and carotene(7).Conclusion 1H-3-aldehyde-indole(1)and N-phenylethylacetamide(5)were obtained from the fermented sophora flavescens for the first time,which may be compounds produced after fermentation.

关 键 词：苦参 内生菌发酵 化学成分 中药转化 

分 类 号：R285.5[医药卫生—中药学]