人胎盘间充质干细胞凝胶促进SD大鼠放射性皮肤损伤的愈合  

Human placenta derived mesenchymal stem cell gel promotes the healing of radiation skin damage in SD rats

作　　者：徐晓明[1] 陈艳[1] 宋倩 袁露 顾加明 张丽娟[2] 耿洁 董坚[1] Xu Xiaoming;Chen Yan;Song Qian;Yuan Lu;Gu Jiaming;Zhang Lijuan;Geng Jie;Dong Jian(Yunnan Tumor Research Institute,The Third Affiliated Hospital of Kunming Medical University/Yunnan Provincial Tumor Hospital/Yunnan Cellular Therapy and Quality Control System Engineering Research Center,Kunming 650118,Yunnan Province,China;Department of Pathology,The Third Affiliated Hospital of Kunming Medical University/Yunnan Provincial Tumor Hospital,Kunming 650118,Yunnan Province,China;Beijing Health-Biotech Group,Beijing 100176,China)

机构地区：[1]昆明医科大学第三附属医院/云南省肿瘤医院肿瘤研究所/云南省细胞治疗及质量控制体系工程研究中心,云南省昆明市650118 [2]昆明医科大学第三附属医院/云南省肿瘤医院病理科,云南省昆明市650118 [3]北京汉氏联合生物技术股份有限公司,北京市100176

出　　处：《中国组织工程研究》2021年第25期3976-3980,共5页Chinese Journal of Tissue Engineering Research

基　　金：云南省细胞治疗技术转化医学重点实验室资助(2015DG034),项目负责人:董坚。

摘　　要：背景:放射性皮肤损伤一直是困扰肿瘤临床放射治疗的难题。大量实验研究显示,人胎盘间充质干细胞具有表皮细胞分化潜能,可促进受损皮肤的愈合。目的:评价局部外用人胎盘间充质干细胞凝胶对大鼠放射性皮肤损伤愈合的影响。方法:取23只SD大鼠,随机分为阴性对照组、空白凝胶组、间充质干细胞凝胶组,其中阴性对照组3只,其余每组10只。各组接受臀背部皮肤照射(采用6 meV电子线,总剂量50 Gy,照射野3 cm×3 cm,单次剂量率500 cGy/min),照射后第23天开始,间充质干细胞治疗组创面均匀涂抹人胎盘间充质干细胞凝胶,空白凝胶对照组创面均匀涂抹空白凝胶,1次/d,连用6次,阴性对照组不进行任何治疗。从第1次用开始每4 d观察1次创面愈合情况,治疗第28天取材,观察人胎盘间充质干细胞凝胶对炎性细胞、创面微血管密度和创面胶原纤维的影响。结果与结论:①阴性对照组照射区皮肤红肿、溃烂;空白凝胶组照射区皮肤僵硬皱缩,观察期内创面均未愈合;间充质干细胞凝胶组照射区长出新生上皮组织,观察期内创面愈合完全;②与阴性对照组和空白凝胶组比较,在治疗后第16,20,24,28天间充质干细胞凝胶组创面愈合速度明显加快(P <0.05),创面面积明显缩小(P <0.05),炎性细胞计数明显减少(P <0.05),微血管计数CD31细胞阳性率明显增多(P <0.05),胶原纤维排列较整齐;③结果表明,局部外用人胎盘间充质干细胞凝胶可促进大鼠放射性皮肤损伤愈合。BACKGROUND:Radiation skin injury has always been a difficult problem in clinical radiotherapy of tumors.A large number of experimental studies have shown that human placenta derived mesenchymal stem cells have the potential for epidermal cell differentiation and can promote the healing of wounded skin.OBJECTIVE:To evaluate the effect of local application of human placenta derived mesenchymal stem cell gel on the healing of radiation skin damage in rats.METHODS:Twenty-three Sprague-Dawley rats were randomly divided into three groups:negative control group(n=3),blank gel group(n=10)and mesenchymal stem cell gel group(n=10).Each group received skin irradiation of buttock and back(6 meV electron wire,total dose of 50 Gy,irradiation field of 3 cm×3 cm,single dose rate of 500 cGy/min).At 23 days after irradiation,the wounds of the mesenchymal stem cell gel group were smeared with human placenta derived mesenchymal stem cell gel.The blank gel control group was smeared with blank gel evenly,once a day,for six times.The negative control group was not given any treatment.The wound healing was observed once every four days.After 28 days of treatment,the effects of human placenta derived mesenchymal stem cell gel on inflammatory cells,wound microvessel density and wound collagen fibers were observed.RESULTS AND CONCLUSION:(1)The skin in the negative control group was swollen and ulcerated;the skin in the blank gel group was stiff and shrunken;and the wounds did not heal during the observation.The mesenchymal stem cell gel group developed new epithelial tissue in the irradiation area,and the wound healing was complete during the observation.(2)Compared with negative control and blank gel groups,at 16,20,24,and 28 days after treatment,the wound healing speed of the mesenchymal stem cell gel group was significantly faster(P<0.05),and wound area was significantly reduced(P<0.05);the count of inflammatory cells was lower(P<0.05);count of CD31 cells in microvessel was significantly higher(P<0.05);collagen fibers were arranged ord

关 键 词：干细胞 人胎盘间充质干细胞凝胶 放射性皮肤损伤 血管化 愈合 炎症 胶原纤维 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R-332[医药卫生—临床医学]