重庆市环境气态污染物暴露与男性精液质量及性激素水平的关联研究  

Association of exposure to environmental gaseous pollutants in Chongqing with semen quality and sex hormone levels in Chongqing young men

作　　者：熊英 王芙蓉 陈卿[1] 杨桓[1] 周文正[2] 张丹[3] 曹佳[1] 周妮娅 XIONG Ying;WANG Furong;CHEN Qing;YANG Huan;ZHOU Wenzheng;ZHANG Dan;CAO Jia;ZHOU Niya(Institute of Toxicology,Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing,400038;Chongqing Health Center for Women and Children,Chongqing,400021;Chongqing Academy of Environmental Sciences,Chongqing,401147,China)

机构地区：[1]陆军军医大学(第三军医大学)军事预防医学系毒理学研究所,重庆400038 [2]重庆市妇幼保健院,重庆400021 [3]重庆市环境科学研究院,重庆401147

出　　处：《第三军医大学学报》2021年第1期10-16,共7页Journal of Third Military Medical University

基　　金：国家自然科学基金重点项目(81630087);国家重点研发计划(2017YFC1002001)。

摘　　要：目的探讨大气气态污染物二氧化硫(SO2)、二氧化氮(NO2)、一氧化碳(CO)和臭氧(O3)等暴露对男性大学生精液质量及性激素水平的影响。方法建立重庆市大学生男性生殖健康研究队列(male reproductive health in Chongqing college students,MARHCS)。于2013年在重庆大学城招募796名男性大学生完成基线调查,并分别在2014、2015年对该人群进行随访,测定精液质量参数、精子DNA损伤以及血清性激素水平。收集研究期间大气污染数据和气象数据,采用混合效应模型分析气态污染物SO2、NO2、O3、CO暴露对精液常规、精子DNA损伤指标以及性激素水平的关联。结果研究对象2013、2014、2015年精子活力和血清孕酮水平比较差异无统计学意义。然而精液量、精子密度、精子总数、精子正常形态率和精子DNA完整性指标高DNA着色性(high DNA stainability,HDS)差异具有统计学意义(P<0.05),血清雌二醇、黄体生成素、泌乳素和睾酮等激素水平差异也有统计学意义(P<0.05)。混合效应模型结果显示:大气SO2暴露与血清泌乳素、孕酮,O3暴露与卵泡刺激素、T/E2比值,NO2与精子正常形态率,CO与卵泡刺激素水平均呈负相关关系,经Bonferroni多重校正后,差异仍有统计学意义(校正P<0.05)。此外,大气O3浓度与精子活力(β=4.88,95%CI:2.60~7.22,校正P<0.05)、精子正常形态率(β=7.28,95%CI:3.44~11.27,校正P<0.05),NO2与卵泡刺激素、黄体生成素和睾酮水平呈正相关关系(校正P<0.05)。结论气态污染物SO2、NO2、O3和CO暴露对男性精液质量及性激素水平可能造成影响。Objective To investigate the effects of atmospheric gaseous pollutants SO2, NO2, CO and O3 on the reproductive endocrine system of male college students. Methods Established cohort of the male reproductive health in Chongqing college students (MARHCS) study. A total of 796 subjects were recruited to complete the baseline survey in 2013, and then followed up in 2014 and 2015. Their semen parameters, sperm DNA damage, and serum sex hormone levels were detected. Air pollution data and meteorological data were also collected during the study period. The mixed effect model was used to analyze the associations of exposure to SO2, NO2, O3 and CO with semen routine parameters, sperm DNA damage indexes and sex hormone levels. Results There were no significant differences in sperm motility and serum progesterone level between the 3 investigations. But statistical differences were observed in semen volume, sperm density, total sperm count, sperm motility and normal sperm morphology, and high DNA stainability (HDS) between the 3 investigations (P < 0.05). So were in the serum levels of estradiol, luteinizing hormone (LH), prolactin and testosterone. The results of the mixed effect model showed that significant negative correlations were seen in SO2 concentration with serum prolactin and progesterone levels, in O3 concentration with follicle stimulating hormone (FSH) and testosterone to estradiol ratio (T/E2), in NO2 with sperm normal morphology rate, and in CO with FSH level. After Bonferroni multiple correction, the differences were still statistically significant (corrected P < 0.05). In addition, O3 concentration was positively correlated with sperm motility (β=4.88;95%CI: 2.60~7.22;corrected P < 0.05) and normal sperm morphology rate (β=7.28;95%CI: 3.44~11.27;corrected P < 0.05). So were the concentration of NO2 with serum FSH, LH and testosterone levels (corrected P < 0.05). Conclusion Exposure to gaseous pollutants SO2, NO2, O3 and CO might affect the semen quality and reproductive hormone levels in young men.

关 键 词：气态污染物 精液质量 血清性激素 MARHCS队列 

分 类 号：R122.7[医药卫生—环境卫生学] R181.34[医药卫生—公共卫生与预防医学]