不同温度对大豆蚜海藻糖合成酶基因表达及沉默效率研究  

Studies on the Expression and Silencing Efficiency of Aphis glycines Trehalose-6-Phosphate Synthase at Different Temperatures

作　　者：杨洪佳 王晓云[1] 车进明 樊东[1] YANG Hongjia;WANG Xiaoyun;JIN Myong Cha;FAN Dong(College of Agronomy,Northeast Agricultural University,Harbin 150030,China;Kyeungsang Sariwon Agricultural University,Pyong Yang 95003,DPR of Korea)

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]桂应相沙里院农业大学,平壤95003

出　　处：《中国生物防治学报》2020年第6期920-928,共9页Chinese Journal of Biological Control

基　　金：现代农业产业技术体系(CARS-04);国家重点研发计划(2018YFD0201000)。

摘　　要：海藻糖是昆虫血淋巴中最重要的糖类物质,海藻糖合成酶(trehalose-6-phosphate synthase,TPS)是昆虫体内海藻糖合成途径的关键酶。本试验通过转录组测序获得一条大豆蚜Aphis glycines(Matsumura)海藻糖合成酶的cDNA序列,命名为AgTPS(GenBank登录号:MN068811)。cDNA序列全长4174 bp,包含一个长度为2559 bp的开放阅读框,编码852个氨基酸,等电点为5.98,分子质量为95.8 kDa,具有一个典型的TPS结构域。不同温度下AgTPS基因干扰结果显示:5℃时基因沉默持续时间最长,30℃处理6 h时该基因沉默效果最好,抑制率可达79.48%,且30℃干扰后对大豆蚜生存和繁殖影响最大。试验表明该基因参与大豆蚜抗逆过程,试验结果为利用RNA干扰技术防治大豆蚜提供理论依据。Trehalose is the most important carbohydrate in insect hemolymph,and trehalose-6-phosphate synthase(TPS)is a key enzyme in the trehalose synthesis pathway in insects.In this study,a cDNA sequence of soybean aphid Aphis glycines(Matsumura)trehalose synthase was obtained through transcriptome sequencing,and named AgTPS(GenBank accession number:MN068811).The cDNA sequence is 4174 bp in length,contains an open reading frame of 2559 bp and encodes 852 amino acids.The isoelectric point of the deduced amino acid is 5.98.The molecular mass is 95.8 kDa.The deduced amino acid has a typical TPS domain.AgTPS was inhibited at different temperatures.It was at 5℃that AgTPS silenced for a long duration.When inhibited at 30℃for 6 h,the gene had the best silence effect and the inhibition rate was 79.48%.The survival and reproduction of soybean aphid were also most affected at 30℃.The results indicated that AgTPS was involved in the stress resistance of soybean aphid.This study provided theoretical basis for the prevention and control of soybean aphid by RNA interference technology.

关 键 词：大豆蚜 海藻糖合成酶基因 温度胁迫 RNA干扰 

分 类 号：S435.65[农业科学—农业昆虫与害虫防治]