ABA对拟南芥AtHsfA1a在冷胁迫响应中生理指标的影响  

Effects of ABA on Physiological Indexes of Arabidopsir AtHsfA1a in Response to Cold Stress

作　　者：姚丽媛 戴利利 陈子牛 郭丽红 YAO Liyuan;DAI Lili;CHEN Ziniu;GUO Lihong(College of Agriculture and Life Sciences,Kunming University,Kunming,Yunnan,China 650214;Key Laboratory of Special Biological Resource Development and Utilization of Universities in Province,Kunming University,Kunming,Yunnan,China 650214;Postgraduate Department,Kunming University,Kunming,Yunnan,China 650214)

机构地区：[1]昆明学院农学与生命科学学院,云南昆明650214 [2]云南省高校特色生物资源开发与利用重点实验室,云南昆明650214 [3]昆明学院研究生处,云南昆明650214

出　　处：《昆明学院学报》2020年第6期59-63,共5页Journal of Kunming University

基　　金：国家自然科学基金资助项目“拟南芥热激因子AtHsfA1a对抗氧化酶的调控”(31060039);国家自然科学基金资助项目“拟南芥热激因子AtHsfA1a对细胞程序性死亡的调控”(31260061);云南省高校特色生物资源开发与利用重点实验室资助项目“拟南芥AtHsfA1a调控抗逆性的作用机制研究”(GXZD201601)。

摘　　要：为从生理水平探讨低温下脱落酸(ABA)与拟南芥热激因子AtHsfA1a的关系,以拟南芥热激因子AtHsfA1a基因T-DNA插入突变型(MT)和野生型(WT)拟南芥为实验材料,研究外源ABA对AtHsfA1a不同基因型拟南芥在低温下的生理指标的影响.结果表明,低温可使幼苗中MDA和H2O2增加,AtHsfA1a基因突变型增加较明显,加入外源ABA后MDA和H2O2有所降低,AtHsfA1a基因突变型降低较少.而抗氧化酶变化趋势正好相反,突变型拟南芥的SOD、CAT、GR和APX活性在低温中比野生型升高小,ABA对冷胁迫中突变型拟南芥抗氧化酶SOD、CAT、GR和APX活性影响较小.说明外源ABA在AtHsfA1a提高抗冷性过程中有调控作用.In order to study the relationship between abscisic acid(ABA) and Arabidopsis heat shock factor AtHsfA1 a at low temperature, Arabidopsis heat shock factor AtHsfA1a gene Mutant of T-DNA insertion(MT)and wild type(WT)of Arabidopsis were used as experimental materials to study the effect of Exogenous ABA on the physiological indexes of different AtHsfA1a genotypes of Arabidopsis seedlings at low temperature. The results showed that the content of MDA and H202 in the Arabidopsismutant increased significantly. The content of MDA and H2O2 decreased after the addition of Exogenous ABA, while that of MDA and H2O2 decreased less in AtHsfA1a gene mutation, but the change trend of antioxidant enzymes was the opposite. The SOD, CAT, GR and APX of Arabidopsis mutant increased less;ABA had little effect on the activity of SOD, CAT, GR and APX of Arabidopsis mutant under cold stress. It is suggested that exogenous ABA can regulate on the process of improving the cold resistance of AtHsfA1 a.

关 键 词：脱落酸 拟南芥热激因子AtHsfA1a 冷胁迫 生理指标 

分 类 号：Q943.2[生物学—植物学]