丝素蛋白负载白藜芦醇微球的制备及其缓释特性  

Preparation of Resveratrol-Loaded Silk Fibroin Microspheres and Study on Its Sustained Release Characteristics

作　　者：张洪铭 曹喜涛[2] 季更生[2] 靳迎春 李强[2] 屠洁[2] Zhang Hongming;Cao Xitao;Ji Gengsheng;Jin Yingchun;Li Qiang;Tu Jie(School of Environmental and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212003,China;School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China)

机构地区：[1]江苏科技大学环境与化学工程学院,江苏镇江212003 [2]江苏科技大学生物技术学院,江苏镇江212018

出　　处：《蚕业科学》2020年第4期522-526,共5页ACTA SERICOLOGICA SINICA

基　　金：镇江市社会发展指导项目(FZ2018075)。

摘　　要：在食品或药物递送领域和缓释领域中,基于丝素蛋白的负载系统因其具备出色的生物相容性和可生物降解性而备受关注。采用自组装法制备平均粒径为242 nm±13.36 nm的丝素蛋白微球,并通过吸附法将性质不稳定的白藜芦醇成功地负载到了丝素蛋白微球中。白藜芦醇的最大包封率和负载率分别为32.41%±0.62%和3.61%±0.02%。体外缓释实验表明,负载白藜芦醇的丝素蛋白微球可以在模拟胃液中稳定持续地释放长达14 d。这体现了丝素蛋白微球在载药及缓释应用中的优越性能。由此可见丝素蛋白微球亦是一种非常有开发价值的药物或食物载体。Loading system prepared by silk fibroin has attracted great attention in the field of delivery and sustained release of drug or food due to its excellent biocompatibility and biodegradability. In this study, silk fibroin microsphere with an average particle size of 242 nm±13.36 nm was prepared by self-assembly, and highly unstable resveratrol was successfully loaded into silk fibroin microsphere by adsorption. The maximum encapsulation efficiency and loading efficiency of resveratrol were 32.41%±0.62% and 3.61%±0.02%, respectively. Moreover, the resveratrol-loaded silk fibroin microsphere can be stably and sustainably released in simulated gastric fluid for 14 d. All above demonstrated that it has superior property of loading and sustained release. Therefore, silk fibroin microsphere is a kind of promising carrier for drug and food.

关 键 词：丝素蛋白 白藜芦醇 自组装 微球 缓释 

分 类 号：S886.9[农业科学—特种经济动物饲养] TQ469[农业科学—畜牧兽医]