牡丹试管苗生根前后的转录组分析  

Transcriptome Analysis on Plantlets before and after Rooting in Vitro of Tree Peony(Paeonia suffruticosa Andr.)

作　　者：符真珠[1] 徐梦岚 袁欣 王慧娟[1] 李艳敏[1] 张和臣[1] Fu Zhenzhu;Xu Menglan;Yuan Xin;Wang Huijuan;Li Yanmin;Zhang Hechen(Horticultural Institute,Henan Academy of Agricultural Sciences,Zhengzhou,450002;College ofForestry,Henan Agricultural University,Zhengzhou,450002)

机构地区：[1]河南省农业科学院园艺研究所,郑州450002 [2]河南农业大学林学院,郑州450002

出　　处：《分子植物育种》2020年第24期8030-8038,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31701945);河南省农业科学院优秀青年基金项目(2020YQ17)共同资助。

摘　　要：牡丹试管苗移栽成活率低的主要原因在于试管苗在生根诱导后发生休眠,导致试管苗弱小,严重影响成活率。为了研究试管苗发生生根休眠的分子机理,本研究对试管苗生根前后(生根培养0, 15 d, 30 d, 45 d)地上部分进行高通量转录组测序,结果显示:测序共获得97.45 Gb Clean Data,各样品Clean Data均达到7.71 Gb,Q30碱基百分比在94.31%以上。组装后共获得43 741条Unigene,其中长度在1 kb以上的Unigene有15 830条。将所有Unigene在NR、Swiss-Prot、KEGG、COG、KOG、GO、Pfam等数据库中进行序列功能注释,共获得26 488条Unigene的注释结果。通过GO分类和KEGG Pathway富集性分析,这些Unigene分别归于43个GO类别和128条代谢途径。不同生根培养时期差异表达基因分析显示,生根培养15 d较生根培养前,有1 416个基因上调,1 170个基因下调;生根培养30 d较生根培养15 d,有1 961个基因上调,1 344个基因下调;而生根培养45 d较生根培养30 d,只有201个基因上调,367个基因下调。这些差异表达基因在KEGG生物通路中,以参与能量物质代谢、植物激素信号转导及次生物质合成等代谢途径富集显著。本研究为进一步挖掘试管苗发生生根休眠的相关调控基因提供理论依据。The main reason for the low transplanting survival rate of plantlet of tree peony is the plantlet ceases growth and is dormant after rooting to lead to the weakness of the plantlets.In order to study the molecular mechanism of dormancy of the plantlet during rooting,the transcriptome sequencing analysis of leaves and stems before and during culture on rooting medium for 0,15,30,and 45 d was carried out by high throughput transcriptome sequencing technology.The results showed that 97.45 Gb Clean Data were obtained in total from the leaves and stems of the plantlets,and the Clean Data of each sample reached 7.71 Gb,Q30 was higher than 94.31%.43741 Unigenes were generated by optimized assembly,among which the length of 15830 Unigenes was more than 1 kb.26488 Unigenes were annotated in NR,Swiss-Prot,KEGG,COG,KOG,GO,Pfam of seven functional databases.The Unigenes were classified into 43 GO categories and 128 KEGG metabolic pathways.Analysis of differentially expressed genes in different stages of rooting revealed that 1416 genes were up-regulated and 1170 genes were down-regulated for 15 days after rooting culture vs before rooting culture.1961 genes were up-regulated and 1344 genes were down-regulated for 30 days vs 15 days after rooting culture.However,only 201 genes were up-regulated and 367 genes were down-regulated for 45 days vs 30 days after rooting culture.These differentially expressed genes related to metabolism of energy substances,plant hormone signal transduction,synthesis of secondary substances and so on significantly enriched in KEGG pathway.This study provided a theoretical basis for further exploring the related regulatory genes of dormant during rooting in vitro of tree peony.

关 键 词：牡丹 试管苗 休眠 转录组 

分 类 号：S685.11[农业科学—观赏园艺]